摘要
目的观察湿润烧伤膏对大鼠肛瘘术后创面模型愈合及创面组织中肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)表达的影响,探讨其促进肛瘘术后创面愈合的作用机制。方法将30只SD雄性大鼠随机分为湿润烧伤膏组、易孚组和生理盐水组,每组10只。3组均模拟建立肛瘘术后创面,造模成功后次日开始换药,湿润烧伤膏组创面局部外敷湿润烧伤膏,易孚组创面局部外敷易孚,生理盐水组创面局部外敷生理盐水。观察3组大鼠换药第3,7,14天创面愈合情况,并计算创面愈合率。换药第14天,HE染色观察创面组织的病理学形态,采用ELISA法检测创面组织中TNF-α、IL-1β含量。结果换药第7天和第14天,湿润烧伤膏组和易孚组大鼠创面愈合率均明显高于同期生理盐水组(P均<0.05),且湿润烧伤膏组均明显高于同期易孚组(P均<0.05)。换药第14天,生理盐水组大鼠创面组织见大量炎症细胞聚集、浸润,少量纤维和胶原蛋白沉积;易孚组和湿润烧伤膏组大鼠创面组织炎性细胞较生理盐水组明显减少,大量胶原蛋白沉积,肉芽组织增多,创面组织中TNF-α、IL-1β含量均明显低于生理盐水组(P均<0.05),且湿润烧伤膏组炎性细胞较易孚组少,胶原组织清晰可见,创面组织中TNF-α、IL-1β含量均明显低于易孚组(P均<0.05)。结论湿润烧伤膏能显著促进大鼠肛瘘术后创面模型愈合,机制可能与下调创面组织中TNF-α、IL-1β的表达,减轻炎症反应相关。
Objective It is to observe the effects of moisture exposed burn ointment(MEBO)on wound healing and the expressions of tumor necrosis factor-α(TNF-α)and interleukin-1β(IL-1β)in wound tissue in rats after anal fistula surgery,and to explore its mechanism in promoting wound healing after the surgery.Methods Thirty male SD rats were randomly divided into MEBO group,Refoo group and normal saline group,with 10 rats in each group.The wound models of postoperative anal fistula were established in the three groups.On the next day after successful modeling,the dressing change was started,the MEBO group was given external application of MEBO on the wound,Yifu group was given external application of Refoo on the wound,and the normal saline group was given external application of normal saline on the wound.The wound healing of rats in the three groups on Day 3,Day 7,Day 14 of dressing change was observed,and the wound healing rates were calculated.On the 14th day of dressing change,the pathological morphology of the traumatized tissues was observed by HE staining,and the contents of TNF-αand IL-1βin the traumatized tissues were detected by ELISA.Results On the 7th day and 14th day of dressing change,the wound healing rates of rats in the MEBO group and Refoo group were significantly higher than those in the normal saline group at the same period(all P<0.05),and the MEBO group was significantly higher than that of the Refoo group at the same period(all P<0.05).On the 14th day of dressing change,a large number of inflammatory cells aggregated and infiltrated with a small amount of fibers and collagen deposition in the traumatic tissue of rats in the normal saline group;the inflammatory cells in the traumatic tissue of rats in the Refoo group and MEBO group were significantly reduced compared with that of the normal saline group,with a large amount of collagen deposition and increase of granulation tissues,and the contents of TNF-αand IL-1βin traumatic tissues were significantly lower than those of the normal saline group(all P<0.05),furthermore,compared with the Refoo group,the inflammatory cells in the MEBO group were fewer,the collagen tissue was clearly visible,and the contents of TNF-αand IL-1βin the traumatic tissue were all significantly lower(all P<0.05).Conclusion Moisture exposed burn ointment can promote wound healing in rats after anal fistula surgery,and the mechanism may be related to down-regulating the expression of TNF-αand IL-1βin wound tissue to relieve inflammatory reactions.
作者
鲍新坤
张晓雨
肖文洁
孙光军
林爱珍
BAO Xinkun;ZHANG Xiaoyu;XIAO Wenjie;SUN Guangjun;LIN Aizhen(Hubei Provincial Hospital of Traditional Chinese Medicine,Wuhan 430061,Hubei,China)
出处
《现代中西医结合杂志》
CAS
2023年第17期2383-2387,共5页
Modern Journal of Integrated Traditional Chinese and Western Medicine
基金
湖北省“十三五”中医重点专科项目(鄂卫办通[2019]125号)
湖北省卫生健康委员会中医药科研青年项目(ZY2021Q034)。
