摘要
马口鱼(Opsariichthys bidens)在我国各大淡水流域分布广泛,是重要的溪流性鱼类,具有重要经济地位。微生物与鱼类的生长和健康状态密切相关。为实现马口鱼的高效健康养殖,本研究从消化道微生物着手,对其消化道菌群总DNA的V3~V4区进行了PCR扩增和建库分析,就其主要微生物组成、分类和相关性等开展分析。结果显示,马口鱼消化道微生物中,芽孢杆菌门(Bacillota)占79.32%,拟杆菌门(Bacteroidota)占4.75%,梭杆菌门(Fusobacteriota)占2.55%,变形菌门(Proteobacteria)占1.69%。在属水平上,支原体属(Mycoplasma)的相对丰度最高,为77.50%,与黄杆菌属(Flavobacterium)和Haliangium的相对丰度表现出显著(P<0.05)负相关。从饲料、养殖水体和滤砂的微生物组成及其相对丰度判断,饲料是马口鱼消化道微生物的主要来源,其次是养殖水体和滤砂,滤砂对水体起到了一定的过滤作用,可基本满足该系统下马口鱼健康养殖的需要。
Being an important stream fish,Opsariichthys bidens is widely distributed in all freshwater basins in China and occupies an important economic position.Microorganisms are closely related to the growth and health of fish.In order to improve the efficient and healthy aquaculture of O.bidens,the PCR amplification of the V3-V4 region of the total DNA in the digestive tract of O.bidens was performed to determine the composition,classification and correlations of the dominant bacteria.The results showed that among the dominant bacteria in the digestive tract of O.bidens,Bacillota,Bacteroidota,Fusobacteriota,Proteobacteria accounted for 79.32%,4.75%,2.55%,1.69%,respectively.At the genus level,the relative abundance of Mycoplasma was the highest,which accounted for 77.50%of the total bacterial genera.The relative abundance of Mycoplasma was significantly(P<0.05)negatively correlated with Flavobacterium and Haliangium.Juged by the abunce of dominant bacteria in feed,aquaculture water and the surface of fliter sand,the main source of bacteria in the digestive tract was feed,which was followed by aquaculture water and filter sand.The sand filter played a certain role in filtering water,which basically met the requirements of healthy aquaculture of O.bidens in this system.
作者
韩明明
詹炜
黄福勇
李方兴
楼宝
HAN Mingming;ZHAN Wei;HUANG Fuyong;LI Fangxing;LOU Bao(Institude of Hydrobiology,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China;Wuyi Xinyi Koi Family Farm,Wuyi 321200,Zhejiang,China)
出处
《浙江农业学报》
CSCD
北大核心
2023年第10期2286-2298,共13页
Acta Agriculturae Zhejiangensis
基金
浙江省重点研发计划(2020C02014,2021C02048)。
关键词
马口鱼
消化道微生物
优势菌
砂滤系统
Opsariichthys bidens
microorganisms in digestive tract
dominant bacteria
sand filter system
