摘要
目的探讨石菖蒲(Acorus gramineus Soland)对铝(Al)致认知功能障碍大鼠学习记忆能力的影响和神经细胞凋亡的抑制情况。方法用氯化铝溶液按照100 mg/kg连续灌胃3个月,建立铝致认知障碍大鼠模型,然后采用石菖蒲灌胃治疗认知。水迷宫行为学实验评估大鼠的学习记忆能力。蛋白免疫印迹法(Western Blot)检测大鼠海马区的凋亡因子B细胞淋巴瘤/2家族蛋白(Bcl-2)、活化的含半胱氨酸的天冬氨酸蛋白水解酶3(Cleaved-Caspase-3)、Bcl-2相关X蛋白(Bax)的蛋白表达水平。实时荧光定量聚合酶链式反应(RT-qPCR)检测Bcl-2、Caspase-3、Bax的mRNA表达水平。结果水迷宫实验结果显示,与模型组相比,药物干预组的大鼠空间探索潜伏期缩短,穿越平台次数显著增多,差异有统计学意义(P<0.05)。Western Blot和RT-qPCR的结果显示,与模型组相比,Bcl-2的表达显著增加,而Bax和Caspase-3的表达显著降低,差异有统计学意义(P<0.05)。结论石菖蒲提高铝致认知功能障碍大鼠的学习记忆能力,降低海马区神经细胞的凋亡。
Objective To investigate the effects of Acorus gramineus Soland on learning and memory ability and the inhibition the apoptosis of nerve cell in rats with cognitive dysfunction induced by aluminum(Al).Methods Continuous intragastrically Al chloride solution of 100 mg/kg for 3 months to established a rat model of cognitive impairment induced by Al,and cognitive impairment was treated by intragastrically Acorus gramineus Soland.The learning and memory ability of rats was evaluated by water maze behavior experiment.The protein expression levels of apoptosis factor B cell lymphoma leukemia-2(Bcl-2),activated cysteinyl aspartate proteolytic enzyme 3(Cleaved-Caspase-3),and Bcl-2 associated X protein(Bax)in the hippocampus of rats were detected by Western Blot.Reverse transcription quantitative real-time polymerase chain reaction(RT-qPCR)was used to detect the mRNA expression levels of Bcl-2,Caspase-3 and Bax.Results The results of water maze experiment showed that the latency of spatial exploration of rats was shortened in the drug intervention group compared with the model group,and the frequency of crossing the platform significantly increased,with statistically significant(P<0.05).The results of Western Blot and RT-qPCR showed that the expression of Bcl-2 was significantly increased and the Bax and Caspase-3 was significantly decreased compared with the model group,with statistically significant(P<0.05).Conclusion Acorus gramineus Soland can improve the learning and memory ability of rats with cognitive dysfunction induced by Al and reduce the apoptosis of nerve cells in the hippocampus.
作者
贾芸菁
李晨羽
甘珏方
廉春容
李莎莎
刘亚林
刘金培
王旭
凌雁武
Jia Yunjing;Li Chenyu;Gan Juefang;Lian Chunrong;Li Shasha;Liu Yalin;Liu Jinpei;Wang Xu;Ling Yanwu(Department of Basic Medicine,Youjiang Medical University for Nationalities,Baise 533000,Guangxi,China)
出处
《右江民族医学院学报》
2023年第5期736-740,共5页
Journal of Youjiang Medical University for Nationalities
关键词
铝
认知功能障碍
石菖蒲
Aluminium
cognitive dysfunction
Acorus gramineus Soland
