摘要
对辣木籽的化学成分进行研究,运用Sephadex LH-20、Toyopearl HW-40F、硅胶、ODS和MCI等柱色谱进行分离、纯化,通过高分辨质谱、^(1)H-NMR、^(13)C-NMR、HMQC、HMBC、^(1)H-^(1)H COSY等波谱法结合化合物理化性质与文献对比,从辣木籽的30%乙醇部位鉴定出12个化合物,分别为乙基-4-O-α-L-吡喃鼠李糖基-α-L-吡喃鼠李糖(1)、乙基-3-O-α-L-吡喃鼠李糖基-α-L-吡喃鼠李糖(2)、(4-羟基苄基)氨基甲酸乙酯(3)、(4-氨基苯基)乙酸(4)、乙基-α-L-吡喃鼠李糖苷(5)、甲基-α-L-吡喃鼠李糖苷(6)、moringapyranosyl(7)、2-[4-(α-L-吡喃鼠李糖基)苯基]乙酸甲酯(8)、niaziridin(9)、5-羟甲基糠醛(10)、对羟基苯乙酰胺(11)和对羟基苯甲酸(12)。其中,化合物1和2为新化合物,化合物3为新天然产物,化合物4~5为首次从辣木属中分离得到。对所有化合物进行了体外α-葡萄糖苷酶的抑制活性评价,其中化合物10具有较好的抑制活性,其IC50为210μg·mL^(-1)。
The chemical constituents of the seeds of Moringa oleifera were isolated and purified by using Sephadex LH-20,Toyo-pearl HW-40F,silica gel,ODS,and MCI column chromatography.The structures of compounds were identified by high-resolution mass spectrometry,^(1)H-NMR,^(13)C-NMR,HMQC,HMBC,and^(1)H-^(1)H COSY,as well as physicochemical properties of compounds and literature data.Twelve compounds were isolated from 30%ethanol fraction of the seeds of M.oleifera and identified as ethyl-4-O-α-L-rhamnosyl-α-L-rhamnoside(1),ethyl-3-O-α-L-rhamnosyl-α-L-rhamnoside(2),(4-hydroxybenzyl)ethyl carbamate(3),(4-aminophenyl)acetic acid(4),ethyl-α-L-rhamnoside(5),methyl-α-L-rhamnoside(6),moringapyranosyl(7),2-[4-(α-L-rhamnosyl)phenyl]methyl acetate(8),niaziridin(9),5-hydroxymethyl furfural(10),4-hydroxybenzeneacetamide(11),and 4-hydroxybenzoic acid(12).Among them,compounds 1 and 2 are two new compounds,compound 3 is a new natural product,and compounds 4-5 were yielded from Moringa plant for the first time.All compounds were evaluated forα-glucosidase inhibitory activity in vitro.Compound 10 showed excellent inhibitory activity with IC_(50)of 210μg·mL^(-1).
作者
陈亮
岑银芝
涂杨丽
戴祥洁
李勇军
杨小生
李林珍
CHEN Liangg;CEN Yin-zhi;TU Yang-li;DAI Xiang-jie;I Yong-jun;YANG Xiao-sheng;LI Lin-zhen(School of Pharmaceutical Sciences,Guizhou Medical University,Guiyang 550025,China;Key Laboratory of Basic Pharmacology of Ministry of Education,Zunyi Medical University,Zunyi 563000,China;Engineering Research Center for the Development and Application of Ethnic Medicine and TCM(Ministry of Education),Guizhou Medical University,Guiyang 550004,China;State Key Laboratory of Functions and Applications of Medicinal Plants,Guizhou Medical University,Guiyang 550004,China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2023年第17期4686-4692,共7页
China Journal of Chinese Materia Medica
基金
国家自然科学基金项目(81860689)
遵义医科大学基础药理教育部重点实验室项目(黔教合KY字[2022]392号)。
关键词
辣木籽
化学成分
糖类化合物
酚苷类化合物
α-葡萄糖苷酶抑制活性
seeds of Moringa oleifera
chemical constituents
carbohydrates
phenolic glycosides
α-glucosidase inhibitory activity
