摘要
目的优化乳胶灌注技术,并将该技术应用于小鼠头面部静脉血管模型制作。方法将9只8周龄、体质量(25.0±1.3)g的雄性C57BL/6小鼠随机分成60%乳胶生理盐水组、60%乳胶肝素组和30%乳胶肝素组,相应试剂灌注完成后将标本浸泡于4℃甲醛固定液中,24h后解剖观察并测量颈外血管直径。结果于小鼠颈外静脉灌注乳胶灌注液200μL后,各组小鼠的眶上静脉、眶下静脉、颞静脉、面后静脉、咬肌静脉、颈外静脉等均得到灌注,通过对灌注后面部血管末端分支、舌下静脉及舌尖小静脉的灌注程度比较后发现,30%乳胶肝素组的灌注效果最佳,其次为60%乳胶肝素组,而60%乳胶生理盐水组的灌注效果最差。结论优化后的乳胶灌注技术可有效灌注小鼠头面部的静脉血管,该技术可为小鼠面部静脉血管的走向及形态研究提供很好的参考。
Objective Optimize the latex perfusion technique and apply it to the construction of a murine craniofacial venous vascular model.Methods A total of nine 8-week-old male C57BL/6 mice weighing(25.0±1.3)g were randomly divided into three groups:60%latex physiological saline group,60%latex heparin group,and 30%latex heparin group.After completion of the perfusion,the specimens were immersed in 4°C formalin fixative for 24 h,followed by dissection,observation,and measurement of the extracranial blood vessel diameters.Results After 200μL latex perfusion solution was injected into the external jugular vein,the supraorbital vein,infraorbital vein,temporal vein,retrofacial vein,masseter vein and external jugular vein were perfused in each group.After comparing the perfusion degree of the distal branches of blood vessels,sublingual vein and tip venule,it was found that the 30%latex heparin group had the best perfusion effect,followed by the 60%latex heparin group,and the 60%latex saline group had the worst perfusion effect.Conclusion The optimized latex perfusion technique can effectively infuse the veins in the head and face of mice,and this technique can provide a good reference for the study of the direction and morphology of facial veins in mice.
作者
王成稷
王珏
王海杰
陆炜晟
史岩
顾正页
万鸣秋
沈如凌
WANG Chengji;WANG Jue;WANG Haijie;LU Weisheng;SHI Yan;GU Zhengye;WAN Mingqiu;SHEN Ruling(Shanghai Laboratory Animal Research Center,Shanghai 201203,China;Mayo Pet(Shanghai)Co.,Ltd.,Shanghai 200235,China)
出处
《实验动物与比较医学》
CAS
2023年第5期574-578,共5页
Laboratory Animal and Comparative Medicine
基金
上海实验动物研究中心科技创新计划新星项目“小鼠肝癌肿瘤血管选择性灌注及透明化技术”(2021NS02)。
关键词
乳胶灌注
血管模型
小鼠头面部血管
Latex perfusion
Vascular model
Craniofacial vessels in mice
