摘要
利用离子色谱-串联质谱技术测定茶叶中氯酸盐、高氯酸盐的含量,通过优化提取条件和色谱-质谱等参数,在茶叶经纯水提取,固相萃取净化后,用离子色谱-质谱负离子模式进行定性、内标法定量分析。结果表明,氯酸盐在0.08~200 ng/mL、高氯酸盐在0.04~100 ng/mL范围内线性良好,相关系数均大于0.999,氯酸盐、高氯酸盐检出限分别1.0、0.5μg/kg,定量限分别为4.0、2.0μg/kg,加标回收率为80.0%~107.9%,相对标准偏差为0.47%~12.3%。所建方法检出限低,精密度良好,可准确定性和定量茶叶中氯酸盐和高氯酸盐的含量,为我国茶叶的风险监测提供了一种有力的技术手段。
The content of chlorate and perchlorate in tea is determined by ion chromatography-tandem mass spectrometry.After optimizing the extraction conditions and the parameters of chromatography-mass spectrometry,the tea samples are first extracted with pure water,followed by purification by solid-phase extraction.Finally,the negative ion mode of ion chromatography-mass spectrometry is used for qualitative analysis and quantitative analysis by internal standard method.The results show that the linearity of chlorate in the range of 0.08~200 ng/mL and perchlorate in the range of 0.04~100 ng/mL are good,and R 2 are higher than 0.999.The detection limits of chlorate and perchlorate are 1.0 and 0.5μg/kg,respectively.The quantitation limits for chlorate and perchlorate are 4.0 and 2.0μg/kg.The recoveries are in range of 80.0%~107.9%and RSDS ranges from 0.47%~12.3%.The method is simple,accurate,sensitive,which can accurately qualitatively and quantitatively determine the content of chlorate and perchlorate in tea,and provides a powerful technical means for the risk monitoring of tea in China.
作者
王娟
谢雨泽
李赞
陈琛
王敬
黄建辉
贾文轩
WANG Juan;XIE Yuze;LI Zan;CHEN Chen;WANG Jing;HUANG Jianhui;JIA Wenxuan(Hebei Food Safety Key Laboratory,Key Laboratory of Special Food Supervision Technology for State Market Regulation,Hebei Engineering Research Center for Special Food Safety and Health,Hebei Food Inspection and Research Institute,Shijiazhuang Hebei 050227,China)
出处
《河北省科学院学报》
CAS
2023年第5期57-63,共7页
Journal of The Hebei Academy of Sciences
基金
河北省市场监督管理局科研计划项目(2021ZD15)。
关键词
草甘膦
氯酸盐
高氯酸盐
离子色谱-质谱
茶叶
Chlorate
Perchlorate
Ion chromatography-tandem mass spectrometry
Tea
