摘要
稀土纳米颗粒(NPs)因能够改善植物生理代谢、促进种子萌发和生长发育及增加产量等有望被应用于农业,但其对植物影响的机制仍不清楚。需在土壤中萌发的植物要经历一段在黑暗中发育的暗形态建成过程,该阶段对于植物后续生长至关重要,但稀土NPs对植物此过程的影响鲜见报道。本文以模式植物拟南芥和纳米氧化镧颗粒(La_(2)O_(3)NPs)分别为植物和稀土NPs的代表,探究不同剂量稀土NPs对植物暗形态建成的影响及机制。结果发现,5, 10, 20和40 mg·L^(-1) La_(2)O_(3)NPs增大拟南芥下胚轴长度、顶端弯钩弯曲角度和原叶绿素酸酯含量,即La_(2)O_(3)NPs促进拟南芥暗形态建成,且在20 mg·L^(-1)时促进作用最大。高效液相色谱和气相色谱结果显示,La_(2)O_(3)NPs处理增加拟南芥赤霉素(GA)、生长素(IAA)和细胞分裂素(ZT)含量及GA/脱落酸(ABA), IAA/ABA, ZT/ABA, GA/乙烯(ETH), IAA/ETH, ZT/ETH,降低ABA和ETH含量,同样20 mg·L^(-1) La_(2)O_(3)NPs处理对拟南芥内源激素影响程度最大。相关性分析表明,La_(2)O_(3)NPs通过增加GA, IAA和ZT含量及GA/ABA, IAA/ABA, ZT/ABA, GA/ETH, IAA/ETH, ZT/ETH,抑制ABA和ETH含量,进而促进拟南芥暗形态建成。为进一步探究La_(2)O_(3)NPs促进暗形态建成的原因,采用实时荧光定量PCR和蛋白质免疫印迹方法测定组成型光形态建成蛋白1 (COP1)和光敏色素互作因子3 (PIF3)的基因相对表达量和蛋白水平,发现La_(2)O_(3)NPs处理增加拟南芥COP1和PIF3基因相对表达量和蛋白水平,推测COP1和PIF3可能参与了La_(2)O_(3)NPs通过内源激素对暗形态建成的调控。本研究可为阐明NPs作用于植物的机制和发展纳米农业提供理论依据。
Rare earth nanoparticles(NPs)are expected to be applied in agriculture due to their ability to im⁃prove plant physiological metabolism,promote seed germination and growth,and increase yield,but the mecha⁃nism of their effects on plants is still unclear.Plants that need to germinate in soil undergo skotomorphogenesis that develops in the dark,which is crucial for subsequent plant growth,but the effect of rare earth NPs on this process has not been reported.In this paper,the model plant Arabidopsisthaliana and La 2 O 3 NPs were used as representatives of plants and rare earth NPs,respectively,to explore the effect and mechanism of different doses of rare earth NPs on skotomorphogenesis of Arabidopsisthaliana.The results show that 5,10,20 and 40 mg·L-1 La 2 O 3 NPs increase hypocotyl length,apical hook angles of curvature and protochlorophyllate content of Arabi⁃dopsisthaliana,that is,La 2 O 3 NPs promote skotomorphogenesis of Arabidopsisthaliana,and 20 mg·L^(-1) La_(2)O_(3) NPs have the greatest promotion effect.The results of high performance liquid chromatography and gas chroma⁃tography show that La_(2)O_(3) NPs treatment increases the contents of gibberellin(GA),auxin(IAA)and cytokinin(ZT)as well as GA/abscisic acid(ABA),IAA/ABA,ZT/ABA,GA/ethylene(ETH),IAA/ETH,ZT/ETH,re⁃duces the contents of ABA and ETH in Arabidopsis thaliana.Similarly,20 mg·L^(-1) La_(2)O_(3) NPs treatment has the greatest effect on Arabidopsis thaliana endogenous hormones.Correlation analysis shows that La_(2)O_(3) NPs promote the skotomorphogenesis of Arabidopsisthaliana by increasing the contents of GA,IAA,ZT and GA/ABA,IAA/ABA,ZT/ABA,GA/ETH,IAA/ETH,ZT/ETH and inhibiting the content of ABA and ETH.Finally,to explore the reason why La_(2)O_(3) NPs promote skotomorphogenesis,real-time quantitative PCR and Western Blot were used to determine the relative gene expressions and protein levels of constitutive photomorphogenesis 1(COP1)and phytochrome-interacting factor 3(PIF3).It is found that La_(2)O_(3) NPs treatment increases the relative gene expres⁃sions and protein levels of COP1 and PIF3 in Arabidopsis thaliana,speculating that COP1 and PIF3 may be in⁃volved in the regulation of skotomorphogenesis by La_(2)O_(3) NPs through endogenous hormones.This study can pro⁃vide a theoretical basis for elucidating the mechanism of NPs acting on plants and developing nano-agriculture.
作者
郭梦蕾
陈琦
代汶昊
马昱
徐梅任
卢博辽
朱云龙
王丽红
Guo Menglei;Chen Qi;Dai Wenhao;Ma Yu;Xu Meiren;Lu Boliao;Zhu Yunlong;Wang Lihong(Jiangsu Key Laboratory of Anaerobic Biotechnology,School of Environment and Civil Engineering,Jiangnan University,Wuxi 214122,China)
出处
《中国稀土学报》
EI
CAS
CSCD
北大核心
2023年第5期986-996,I0004,共12页
Journal of the Chinese Society of Rare Earths
基金
国家自然科学基金项目(21501068)资助。
