摘要
【目的】优化一株盐碱绿藻的SE培养基,为盐碱藻分子生物学资源的研究提供理论依据。【方法】对塔里木大学校园内东湖中分离纯化的一株盐碱绿藻进行培养基筛选,通过形态观察及分子鉴定,研究CaCl_(2)·2H_(2)O、KH_(2)PO_(4)、NaCl、NaNO_(3)、MgSO_(4)·7H_(2)O以及pH对其生长的影响,并进行系统发育分析。【结果】盐碱绿藻为椭圆形藻。CaCl_(2)·2H_(2)O最适浓度为0.050 g/L,可溶性糖为24.34%,可溶性蛋白为16.55%,总叶绿素为2.000 g/L。KH_(2)PO_(4)最适浓度为0.175 g/L,可溶性糖为10.53%,可溶性蛋白为7.7%,总叶绿素为3.607 g/L。NaCl最适浓度为0.100 g/L,可溶性糖为11.82%,可溶性蛋白为8.57%,总叶绿素为3.385 g/L。NaNO_(3)最适浓度为0.250 g/L,可溶性糖为10.64%,可溶性蛋白为6.10%,总叶绿素为2.731g/L。MgSO_(4)·7H_(2)O最适浓度为0.375 g/L,可溶性糖为12.32%,可溶性蛋白为9.9%,总叶绿素为1.790 g/L。pH最适浓度为9.0,可溶性糖为16.57%,可溶性蛋白为9.46%,总叶绿素为1.985 g/L。1号藻的序列与新颖拟绿球藻属(Pseudochlorococcum sp.)聚簇成一支亲缘关系很相近。【结论】确定了1号盐碱绿藻的分类地位以及SE培养基优化后CaCl_(2)·2H_(2)O的浓度为0.050 g/L,KH_(2)PO_(4)的浓度为0.175 g/L,NaCl的浓度为0.100 g/L,NaNO_(3)的浓度为0.250 g/L,MgSO_(4)·7H_(2)O的浓度为0.375 g/L和pH为9.0。
【Objective】Optimization of SE medium of a saline-alkali green algae,the purpose of this study is to provide a theoretical basis for the research on the molecular biology and ecological environment protection of saline-alkali green alga as well as fishery bait resources.【Methods】A saline-alkali green alga isolated and purified from East Lake of Tarim University is screened for culture medium,morphological observation and molecular identification.Investigate the effects of CaCl_(2)·2H_(2)O,KH_(2)PO_(4),NaCl,NaNO_(3),MgSO_(4)·7H_(2)O and pH on its growth,and conduct phylogenetic analysis.【Results】The morphological characteristics showed that the algae is ellipse.The optimum concentration of CaCl_(2)·2H_(2)O is 0.050 g/L,the soluble sugar is 24.34%,the soluble protein is 16.55%,and the total chlorophyll is 2.000 g/L.The optimum concentration of KH_(2)PO_(4)is 0.175 g/L,the soluble sugar is 10.53%,the soluble protein is 7.7%,and the total chlorophyll is 3.607 g/L.The optimum concentration of NaCl is 0.100 g/L,the soluble sugar is 11.82%,the soluble protein is 8.57%,and the total chlorophyll is 3.385 g/L.The optimum concentration of NaNO_(3)is 0.250 g/L,the soluble sugar is 10.64%,the soluble protein is 6.10%,and the total chlorophyll is 2.731 g/L.The optimum concentration of MgSO_(4)·7H_(2)O is 0.375 g/L,the soluble sugar is 12.32%,the soluble protein is 9.9%,and the total chlorophyll is 1.790 g/L.The optimum pH concentration is 9.0,the soluble sugar is 16.57%,the soluble protein is 9.46%,and the total chlorophyll is 1.985 g/L.The results showed that the sequence of No.1 algae is closely related to the novel Pseudochlorococcum sp.【Conclusion】This study determined the classification status of the saline No.1 and the concentration of CaCl_(2)·2H_(2)O,KH_(2)PO_(4),NaCl and NaNO_(3)after SE medium optimization was 0.050 g/L,0.175 g/L,0.100 g/L,0.250 g/L,respectively.The concentration of MgSO_(4)·7H_(2)O is 0.375 g/L and the pH is 9.0.
作者
陈凤
董宇
蔡宏宇
张苏江
雷曼红
孙禹
CHEN Feng;DONG Yu;CAI Hongyu;ZHANG Sujiang;LEI Manhong;SUN Yu(Tarim University,Xinjiang Production and Construction Corps Tarim Animal Husbandry Science and Technology Key Laboratory,Aral Xinjiang 843300,China)
出处
《新疆农业科学》
CAS
CSCD
北大核心
2023年第10期2509-2520,共12页
Xinjiang Agricultural Sciences
基金
国家自然科学基金项目(31660738)。
关键词
盐碱绿藻
18S
rDNA
形态特性
培养基优化
saline-alkali green alga
18S rDNA
morphological properties
medium optimization
