摘要
以带腋芽茎段为试验材料,采用不同浓度和种类的试剂及培养基进行组培的方法,研究杀菌剂、培养基、生长激素对外植体灭菌、芽萌发、组培苗生根和移栽的影响,为构建全面、高效的‘缤纷秋色’快繁体系提供参考。结果表明,外植体最佳灭菌方法为10%NaClO溶液消毒12 min,污染率减少至19.6%,萌芽数为8.76个。最适启动培养基为WPM+0.2 mg/L NAA+1.0 mg/L 6-BA+0.2 mg/L TDZ+2.0 mg/L PVP+30 g/L蔗糖;壮苗培养基为WPM+0.8 mg/L ZT+1.0 mg/L 6-BA+2.0 mg/L PVP+30 g/L蔗糖;生根培养基为1/2WPM+0.8 mg/L IBA+0.2 mg/L NAA+0.5 g/L活性炭+15 g/L蔗糖,用草炭土和珍珠岩混合比例2:1移栽效果最好,移栽成活率为89%。研究建立的‘缤纷秋色’快速繁殖体系可为优良种质扩大培养和推广应用提供技术参考。
The stem segment of axillary bud were used as experimental materials,the effects of fungicides,medium and growth hormone on explant sterilization,cluster bud induction,rooting and transplantation of tissue culture were studied by tissue culture method with different concentration and types of agents,culture media and hormone,to provide reference for the construction of comprehensive and efficient rapid propagation system of Acer truncatum×A.platanoides‘Warrenred’system.The results showed that the best sterilization method for explants was 10%NaClO solution disinfection for 12 minutes,the pollution was reduced to 19.6%,and the number of germination was 8.76.The optimum medium for bud induction was WPM+0.2 mg/L NAA+1.0 mg/L 6-BA+0.2 mg/L TDZ+2.0 mg/L PVP+30 g/L sucrose;the optimum combination of strong culture was WPM+0.8 mg/L ZT+1.0 mg/L 6-BA+2.0 mg/L PVP+30 g/L sucrose;1/2WPM+0.8 mg/L IBA+0.2 mg/L NAA+0.5 g/L AC+15 g/L sucrose was available to culture rooting.The best transplanting substrate was mixture of humus soil:vermiculite=2:1,the survival rate was maximal for 89%.This study established a rapid propagation system for the shoots of Acer truncatum×A.platanoides‘Warrenred’,and could lay a foundation for the subsequent transplanting,culture expanding and popularizing of the fine germplasms.
作者
蒋淑磊
白霄霞
刘雅
赵兴兴
郑书海
陈霞
JIANG Shulei;BAI Xiaoxia;LIU Ya;ZHAO Xingxing;ZHENG Shuhai;CHEN Xia(Shijiazhuang Academy of Agriculture and Forestry Sciences/Engineering Research Center for Floriculture,Shijiazhuang 050041;Shijiazhuang Shenghe Agricultural Science and Technology Co.Ltd.,Shijiazhuang 050031;Hebei Academy of Forestry and Grassland Science,Shijiazhuang 050061)
出处
《中国农学通报》
2023年第28期7-11,共5页
Chinese Agricultural Science Bulletin
基金
石家庄科技支撑项目“优质高抗杂交挪威槭-缤纷秋色繁育关键技术研究”(211520172A)
石家庄科技支撑项目“彩叶树种槭树抗寒种质资源创制及新品种选育”(221520122A)。
关键词
挪威槭
‘缤纷秋色’
组培快繁
再生体系
植株再生
Acer platanoides
Acer truncatum×A.platanoides‘Warrenred’
tissue culture
regenerative system
plant regeneration
