摘要
试验旨在筛得耐盐耐低温可调水的光合细菌并进行扩培培养,为光合细菌的产业化生产及应用提供参考。试验由环境样中分离光合细菌,经耐盐性低温试验及降亚硝能力测试筛菌,采用响应面优化筛得菌株培养基。结果显示,DBNRh06在光照及黑暗条件下培养48 h时对亚硝酸盐降解率均可达65%,在盐度为35条件下的OD660 nm值可达0.7,在15℃、120 h时对亚硝酸盐的降解率仍可达50%。利用响应面优化法优化DBNRh06得到最佳培养基配方为草酸铵2 g/L、乙酸钠1.5 g/L、酵母粉1.5 g/L。利用最优培养基配方,在30℃、1000~2000 lx光照培养96 h,得到DBNRh06的OD_(660 nm)值为0.877,与预测的最大值0.860非常接近。研究表明,筛得1株耐盐度较好且低温仍可降亚硝酸盐的沼泽红假单胞菌,为光合细菌在水产养殖业的实际应用奠定基础。
The aim of the experiment was to screen and expand the cultivation of salt-tolerant and low-temperature water-regulating photosynthetic bacteria,and provide reference for the industrial production and application of photosynthetic bacteria.Photosynthetic bacteria were isolated from environmental samples,screened by low temperature test of salt tolerance and nitrite reduction ability,and screened by response surface optimization.The results showed that the nitrite degradation rate of DBNRh06 could reach 65%under both light and dark conditions for 48 h,OD660 nm could reach 0.7 under salinity of 35,and the nitrite degradation rate could still reach 50%at 15℃and 120 h.The optimal medium formula of DBNRh06 was ammonium oxalate 2 g/L,sodium acetate 1.5 g/L and yeast powder 1.5 g/L.Using the optimal medium formula,the OD660 nm of DBNRh06 was 0.877,which was very close to the predicted maximum value of 0.860,after being cultured at 30℃and 1000~2000 lx for 96 h.The study indicates that a strain of Rhodopseudomonas palustris with good salinity tolerance and low temperature can still reduce nitrite is screened,which has a foundation for the practical application of photosynthetic bacteria in aquaculture industry.
作者
鲁晏宏
王蕊
王珺
胡婷
付维来
朱传忠
汪攀
LU Yan-hong;WANG Rui;WANG Jun;HU Ting;FU Wei-lai;ZHU Chuan-zhong;WANG Pan
出处
《饲料研究》
CAS
北大核心
2023年第20期103-108,共6页
Feed Research
基金
福建省“百人计划”创新人才项目
九江市“双百双千”项目
中央引导地方科技发展专项“大黄鱼健康养殖及主要病害综合防控技术集成创新与产业化(项目编号:2022L3020047)”。
关键词
沼泽红假单胞菌
培养基优化
响应面优化
Rhodopseudomonas palustris
medium optimization
response surface method
