摘要
通过检测26个StIAA家族基因在马铃薯根系发育过程中的表达发现:18个StIAA基因在根系成熟后的表达量比发育初期显著上调,8个StIAA基因变化不显著。利用人工microRNA(artificial microRNA,amiRNA)技术对上调表达最为显著的StIAA22做进一步的功能研究:构建干扰表达载体pBI121-amiRIAA,通过根癌农杆菌介导法转入马铃薯栽培品种‘Dèsirèe’。经定量PCR检测,StIAA22在所有转基因株系中的表达均受到明显抑制;其根系形态构型与非转基因植株差异明显,根系生长受到抑制,根长明显变短,侧根数量增加,根系生物量减少。以上结果表明StIAA22在调节马铃薯根系形态建成中起关键作用。
Quantitative real-time PCR(qRT-PCR)were used to analyze the expression levels of 26 StIAA genes during potato root development,the results showed that 18 StIAAs were obviously up-regulated between the early period and the mature period of potato development,expression level of other eight StIAAs are not obvious.In addition,artificial microRNA(amiRNA)were used to futher functional study of StIAA22 that is one of the most significant up-regulated StIAAs.Interference vector of pBI121-amiRIAA was constructed,and transferred into the potato genome mediated by Agrobactium tumefacienses to obtain the transgenic plants.The qRT-PCR analysis showed that the expression of StIAA22 was obviously restrained in all transgenic plant lines.Also,the transgenic lines showed a drastic change in root architecture compared with that in control plants by suppressing StIAA22 expression,such as severe reduction in the length of root,an increase in number of lateral roots,and a decrease in biomass of potato roots.The results showed that StIAA22 had a key role in modulating potato root architecture.
作者
杨江伟
段小琴
张飞燕
张丽
邓玉荣
王晓凤
郑智勇
张宁
司怀军
YANG Jiangwei;DUAN Xiaoqin;ZHANG Feiyan;ZHANG Li;DENG Yurong;WANG Xiaofeng;ZHENG Zhiyong;ZHANG Ning;SI Huaijun(State Key Laboratory of Aridland Crop Science,Gansu Agricultural University,Lanzhou 730070,China;College of Life Science and Technology,Gansu Agricultural University,Lanzhou 730070,China;Agricultural Biotechnology Research Center,Ningxia Academy of Agricultural and Forestry Sciences,Yinchuan 750002,China)
出处
《园艺学报》
CAS
CSCD
北大核心
2023年第10期2091-2103,共13页
Acta Horticulturae Sinica
基金
国家自然科学基金项目(31860400)
甘肃农业大学青年研究生指导教师扶持基金项目(GAU-QDFC-2021-15)
省部共建干旱生境作物学国家重点实验室基金项目(GSCS-2017-6)
甘肃省科技重大专项计划项目(GZGG-2021-6)。
