摘要
目的研究雷公藤Tripterygium wilfordii的化学成分及其黄烷醇类化合物抗氧化活性。方法利用硅胶色谱柱、中压制备液相、半制备液相等色谱技术进行分离纯化,通过NMR光谱并结合文献报道的数据对化合物进行鉴定,并通过DPPH自由基的清除能力为评价指标对其黄烷醇类单体化合物体外抗氧化活性进行评估。结果从雷公藤的干燥根茎中分离得到12个化合物,分别鉴定为右旋儿茶素(1)、左旋儿茶素(2)、左旋表阿福豆素(3)、右旋阿福豆素(4)、雷酚二萜酸(5)、雷藤二萜醌A(6)、壬二酸(7)、整合素A(8)、(S)-5-(3,4-二羟基苯基)-γ-缬草内酯(9)、茶曲霉酚(10)、雷藤二萜醌F(11)、冬青卫矛倍半萜4(12)。化合物1~4对DPPH自由基的半数清除率(half inhibitory concentration,IC_(50))分别为1.29、0.75、0.81、1.49 mmol/L。结论化合物3~4、7~10为首次从该植物中分离得到。化合物1~4清除DPPH自由基能力较强,具有良好的抗氧化活性。
Objective To investigate the chemical constituents and the antioxidant activities of flavanols from the roots of Tripterygium wilfordii.Methods The ethonal extracts of T.wilfordii were purified by comprehensive chromatography,such as silica gels,medium pressure liquid chromatography(MPLC),semi-preparative HPLC,and the structures of purifie compounds were identified based on spectroscopic and compared with data from the literatures.The antioxidant activities of the flavanol monomeric compounds from polar extracts were evaluated by DPPH·scavenging assay.Results Twelve compounds were isolated and identified as(+)-catechin(1),(−)-catechin(2),(−)-epi-afzelechin(3),(+)-afzelechin(4),triptonoditerpenic acid(5),triptoquinone A(6),azelaic acid(7),integracin A(8),(S)-5-(3,4-dihydroxyphenyl)-γ-valerolactone(9),teasperol(10),triptoquinone F(11)and ejap 4(12).Among them,compounds 1—4 had stronger DPPH free radicals scavenging ability,with IC_(50) values of 1.29,0.75,0.81,1.49 mmol/L,respectively.Conclusion Compounds 3—4,7—10 are isolated from T.wilfordii for the first time.In vitro analyses indicated that compounds 1—4 possess high free radical scavenging ability and effective anti-oxidant activity.
作者
黎曼欣
崔夏莲
黄冬冬
纪瑞锋
杨继
何新
LI Man-xin;CUI Xia-lian;HUANG Dong-dong;JI Rui-feng;YANG Ji;HE Xin(School of Traditional Chinese Medicine,Guangdong Pharmaceutical University,Guangzhou 510006,China)
出处
《中草药》
CAS
CSCD
北大核心
2023年第19期6220-6227,共8页
Chinese Traditional and Herbal Drugs
基金
广东省自然科学基金项目(2020A1515010156)
国家自然科学基金资助项目(82174209)
广东省重点学科科研项目(2019-GDXK-0014)。
