摘要
目的研究松杉灵芝中总三萜的最佳提取工艺。方法采用乙醇回流提取,以松杉灵芝内三萜类化合物的含量为指标,通过单因素结合正交试验考察松杉灵芝的最佳提取工艺。结果影响松杉灵芝三萜回流提取的因素顺序为:乙醇浓度>料液比>提取时间,乙醇浓度和料液比具有显著性,70%乙醇浓度和1∶14料液比提取效果最佳,提取时间不具有显著性,提取1.5 h效果最佳。结论松杉灵芝最佳的提取工艺为14倍量70%的乙醇回流提取2次,每次1.5 h,该工艺较为稳定,具有简单、可行的特点。
Objective To study the optimal extraction process of total triterpenes from Ganoderma tsugae.Methods Ethanol reflux extraction was used,with the content of triterpenoids in Ganoderma tsugae as the indicator.The optimal extraction process of Ganoderma tsugae was investigated through single factor combined with orthogonal experiments.Results The order of factors affecting the reflux extraction of triterpenoids from Ganoderma tsugae is as follows:ethanol concentration>material liquid ratio>extraction time.The ethanol concentration and material liquid ratio have significant effects,while the 70%ethanol concentration and 1∶14 material liquid ratio have the best extraction effect.The extraction time is not significant,and the best effect is obtained after 1.5 hours of extraction.Conclusion The optimal extraction process for Songshan Ganoderma tsugae is 14 times the amount of 70%ethanol reflux extraction twice,each time for 1.5 hours.This process is relatively stable and has the characteristics of simplicity and feasibility.
作者
杨潺
王晓明
雷钧涛
王岩
YANG Chan;WANG Xiaoming;LEI Juntao;WANG Yan(Changchun University of Chinese Medicine,Changchun,Jilin Province,130117;Jilin Municipal Hospital of T.C.M,Jilin City,Jilin Province,132013;Jilin Medical University,Jilin City,Jilin Province,132013,China)
出处
《吉林医药学院学报》
2023年第6期410-412,共3页
Journal of Jilin Medical University
基金
吉林省中医药科技支持项目(2023101)。
关键词
松杉灵芝
三萜
醇提工艺
单因素实验
正交试验
Ganoderma tsugae
triterpenes
alcohol extraction process
single factor test
orthogonal test
