摘要
为解析平菇发酵培养料制备过程中黄曲霉毒素降解的变化规律,采用非靶向代谢组学技术和宏基因组学技术,对平菇发酵培养料制备过程中5个时期黄曲霉毒素含量及相关微生物基因丰度进行关联分析。结果表明,发酵初期(T1)培养料含有AFM1、AFG2两种黄曲霉毒素,升温阶段(T2)其含量均升至最高;第2次至第4次翻堆(T3至T5)时期未检测到黄曲霉毒素。相关性分析表明,两种黄曲霉毒素含量与曲霉属的相对丰度呈显著正相关。类芽胞杆菌属(Paenibacillus)和藤黄单胞菌属(Luteimonas)在发酵过程中是优势属,且具有降解黄曲霉毒素的能力;发酵过程中黄曲霉毒素含量变化与该2个属的相对丰度呈极显著负相关,推测该2个属可能在平菇培养料发酵过程中降解了黄曲霉毒素。
In order to analyze the change of the degradation of aflatoxins during the compost substrate preparation process of Pleurotus ostreatus,non-targeted metabolomics and metagenomic sequencing techniques were used to analyze aflatoxin content and related microbial gene abundance in 5 periods during the compost process.The results showed that in the initial stage of compost(T1),the substrates contained two types of aflatoxins,AFM1 and AFG2,while in the temperature increasing stage(T2),their contents rose to the highest.No aflatoxin was detected during the second to fourth turnover(T3 to T5)period.Correlation analysis showed that the content of two aflatoxins were significantly positively correlated with the relative abundance of Aspergillus genus during the compost process.Paenibacilli and Luteimonas were dominant genera in the compost process and had the ability to degrade aflatoxin;it was found that there was a highly significant negative correlation between the aflatoxin content and the relative abundance of these two genera during the compost process by correlation analysis.Therefore,it is speculated that these two genera may have degraded aflatoxin during the compost process.
作者
崔筱
胡素娟
刘芹
吴杰
师子文
张玉亭
孔维丽
CUI Xiao;HU Sujuan;LIU Qin;WUJie;SHI Ziwen;ZHANG Yuting;KONGWeili(Institute of Edible Fungi,Henan Academy of Agricultural Sciences,Zhengzhou 450002,Henan,China)
出处
《中国瓜菜》
CAS
北大核心
2023年第11期93-99,共7页
China Cucurbits And Vegetables
基金
河南省现代农业产业技术体系专项(HARS-22-08-S,HARS-22-08-G1)
河南省重大公益性专项(201300110700)。
关键词
平菇
发酵培养料
黄曲霉毒素
降解
Pleurotus ostreatus
Compost substrate
Aflatoxin
Degradation
