摘要
运用大孔树脂、硅胶、ODS和Sephadex LH-20等柱色谱,结合半制备液相、TLC等分离方法对红簕钩80%乙醇提取物的乙酸乙酯部位分离纯化得到8个三萜类化合物。通过1D/2D NMR、MS、UV、IR等波谱数据和与文献对比确定化合物的结构,分别鉴定为悬钩子裂环三萜A(1)、悬钩子裂环三萜B(2)、号角树酸(3)、号角树酸-3-甲酯(4)、麦珠子酸(5)、白桦酯酸(6)、白桦脂醇(7)和obtusalin(8)。其中,化合物1、2为新化合物,化合物3~8为首次从该植物中分离得到。
Eight compounds were isolated from the ethyl acetate fraction of the 80%aqueous ethanol extract of the roots and stems of Rubus pirifolius Smith by AB-8 macroporous resin,silica gel,ODS,Sephadex LH-20 column chromatography,and semi-preparative HPLC.Their structures were identified by spectral analysis such as 1D/2D NMR,MS,UV,IR and by comparison with literature information as rubussecotriterpene A(1),rubussecotri-terpene B(2),cecropiacic acid(3),cecropiacic acid 3-methyl ester(4),alphitolic acid(5),betulinic acid(6),betulin(7),and obtusalin(8).Compounds 1 and 2 are new compounds,and compounds 3-8 were isolated from this plant for the first time.
作者
宁小燕
黄美芳
梁沁沁
冯旭
梁臣艳
奉建芳
王柳萍
韦建华
NING Xiao-yan;HUANG Mei-fang;LIANG Qin-qin;FENG Xu;LIANG Chen-yan;FENG Jian-fang;WANG Liu-ping;WEI Jian-hua(College of Pharmacy,Guangxi University of Chinese Medicine,Nanning 530200,China;Key Laboratory of TCM Extraction and Purification and Quality Analysis,Education Department of Guangxi Zhuang Autonomous Region,Nanning 530200,China;Guangxi Superior Chinese Patent Medicine and National Medicine Development Engineering Technology Research Center,Nanning 530200,China)
出处
《药学学报》
CAS
CSCD
北大核心
2023年第10期3065-3069,共5页
Acta Pharmaceutica Sinica
基金
国家重点研究计划项目(2019YFC1712304)
广西科技基地和人才专项(桂科AD20238058)
广西中医药管理局科研课题(GXZYZ20210068)
广西壮瑶药重点实验室项目(GXZYKF2022-16)
广西中医药大学科研项目(2020ZD002)
广西中医药大学研究生教育创新计划项目(YCXJ2021020)
广西中医药大学一方制药大学生科技创新课题项目(2021DXS44)
国家级大学生创新创业训练计划项目(202110600013)。
关键词
红簕钩
2
3-裂环三萜
化学成分
Rubus pirifolius Smith
2,3-seco triterpene
chemical composition
