摘要
目的探讨卵巢中结缔组织生长因子(CTGF)及基质金属蛋白酶-9(MMP-9)的表达与多囊卵巢综合征的关系。方法52只SPF级SD雌性大鼠(6周龄,具备规律动情周期)依照不同造模方式分为对照组和模型组,每组26只。对照组行羧甲基纤维素灌胃建模,模型组行等量来曲唑建模。分析研究对象动情周期不在出现固定规律,且具有连续性特征时,两组均采集其血清以及卵巢组织,采用放射免疫法(RIA)测定促黄体生成激素(LH)、促卵泡激素(FSH)、雌二醇(E_(2))、垂体泌乳素(PRL)、睾丸素等指标对大鼠内分泌改变情况进行评定分析;并应用免疫组织化学的方法使用CTGF一抗、二抗、三抗和MMP-9一抗、二抗、三抗来测定和分析两种因子在该疾病组织中的表达情况。结果模型组PRL、LH、睾丸素、窦前卵泡卵泡膜胞浆中CTGF、黄体中MMP-9等表达高于对照组(P<0.05),FSH、E_(2)、卵泡基底膜内MMP-9表达水平均发生显著下降趋势(P<0.05)。结论卵巢中结缔组织生长因子水平变化与该疾病中小卵泡过多表达或者排卵障碍之间存在一定关联性,MMP-9则与多囊卵巢综合征的排卵障碍之间存在关联性。
Objective To study the relationship between the expression of connective tissue growth factor(CTGF)and matrix metalloproteinase-9(MMP-9)in ovary and polycystic ovarian syndrome.Methods 52 female rats(6 weeks of age,with regular estrous cycles)were divided into two groups(control group and model group)according to different modeling methods.The control group was given carboxymethyl cellulose by gavage,and the model group was given the same amount of letrozole.The serum and ovarian tissue of the two groups were collected when the estrous cycle of rats was not fixed and had the characteristics of continuity.RIA method was used to determine LH,FSH,E2,PRL,and testosterone,respectively.The endocrine changes of model mice were evaluated and analyzed by PRL,testosterone and other indexes.The expression of connective tissue growth factor and MMP-9 in ovarian tissues was determined and analyzed by immunohistochemical methods using CTGF primary antibody,secondary antibody,third antibody and MMP-9 primary antibody,secondary antibody and third antibody.Results The expression levels of PRL,LH,presinus follicular cytoplasm(CTGF,albumen CTGF,basal membrane cytoplasm of follicle CTGF)and MMP-9 in corpus luteum of the model group were significantly higher than those of the control group(P<0.05).However,the expression levels of FSH,serum estradiol and MMP-9 in the basal membrane of follicles were significantly decreased(P<0.05).Conclusion There is a certain correlation between the level of connective tissue growth factor in the ovary and the overexpression of small and medium follicles or ovulation disorder in this disease,and MMP-9 is associated with ovulation disorder in polycystic ovary syndrome.
作者
张璐
刘明盛
杨旭
李兰
ZHANG Lu;LIU Mingsheng;YANG Xu;LI Lan(Department of Obstetrics and Gynecology,The Fifth People′s Hospital Affiliated to Chengdu University of Traditional Chinese Medicine,Chengdu 611130,China)
出处
《西部医学》
2023年第11期1589-1593,共5页
Medical Journal of West China
基金
四川省科技厅重点项目(22MZGC0259)。
