摘要
为建立一种同时检测茶叶样品中农药多残留的分析方法,本研究以亲水型低共熔溶剂(HP-DES)作为分散液液微萃取(DLLME)的萃取剂,对茶叶样品中的农药进行萃取富集,再通过高效液相色谱-串联质谱法(HPLC-MS/MS)对农药残留量进行分析测定。结果表明,茶叶样品经过HP-DES提取,涡旋离心后能够有效去除杂质,富集目标物。茶叶中65种目标农药在1~500 ng·mL^(-1)范围内线性关系良好,线性关系系数(r^(2))均大于0.996,定量限为0.12~9.76μg·kg^(-1),3个添加浓度水平(10、50、100μg·kg^(-1))下的平均回收率在64.44%~110.15%之间,其相对标准偏差(RSD)均小于15%。本方法快捷简便、绿色环保、成本低且性能良好,为茶叶基质中农药残留的检测提供了技术支持。
In order to establish an analytical method for simultaneous detection of multiple pesticide residues in tea samples,the hydrophilic deep eutectic solvent(HP-DES)was used as the extractant of the dispersive liquid-liquid microextraction(DLLME)to extract and enrich pesticides in tea samples,and pesticide residues were analyzed by high-performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS).The results showed that impurities in the tea samples could be effectively removed and the target substances were enriched after HP-DES extraction,vortex and centrifugation.The calibration curves of 65 pesticide residues had good linearity in the range of 1~500 ng·mL^(-1),the correlation coefficient(r^(2))was larger than 0.996,and the limits of quantification(LOQ)ranged from 0.12 to 9.76μg·kg^(-1).In addition,the average recoveries were in the range of 64.44%to 110.15%at three spiked levels(10μg·kg^(-1),50μg·kg^(-1) and 100μg·kg^(-1))and the relative standard deviation(RSD)was lower than 15%.It is a fast,simple,green,low cost method with good performance,which provides technical support for the detection of pesticide residues in tea matrix.
作者
陈文辉
王华威
李建勋
谭海广
李倩倩
杨术鹏
杜丽平
李熠
CHEN Wenhui;WANG Huawei;LI Jianxun;TAN Haiguang;LI Qianqian;YANG Shupeng;DU Liping;LI Yi(College of Biotechnology,Tianjin University of Science and Technology,Tianjin 300457;Institute of Agricultural Products Processing Sciences and Technology,Chinese Academy of Agricultural Sciences,Beijing 100193)
出处
《核农学报》
CAS
CSCD
北大核心
2023年第9期1823-1832,共10页
Journal of Nuclear Agricultural Sciences
基金
国家自然科学基金资助项目(32202183)。
