摘要
以亚临界水降解高温芝麻饼粕蛋白水解液为研究对象,通过纳滤、超滤及液相层析系统对水解液中血管紧张素转化酶(angiotension converting enzyme,ACE)抑制肽进行分离纯化,并通过液相色谱-质谱联用仪进行结构鉴定,合成相应寡肽验证ACE抑制活性。预测寡肽的吸收代谢特性,建立三维定量构效关系(three dimensional quantitative structure-activity relationship,3D-QSAR)模型,并进行分子对接分析。结果显示:分子质量<3 kDa组分具有最强的ACE抑制作用,进一步纯化后从峰1组分中共鉴定到9个ACE抑制肽,这些肽不会干涉人体正常的生理活动。基于比较分子场分析法成功建立LFRAF的3D-QSAR模型。ACE抑制肽的C端处带正电荷氨基酸残基与侧链处引入大基团能够提高ACE抑制能力。LFRAF通过占据ACE的S2、S1′活性口袋,并与Zn2+结合从而抑制ACE活性。该结果表明采用亚临界水技术降解高温芝麻粕中蛋白制备ACE抑制肽可行。
Angiotension converting enzyme(ACE)inhibitory peptides from high-temperature sesame meal protein hydrolyzed by subcritical water were separated and purified by sequential nanofiltration,ultrafiltration and liquid chromatography,and their structures were identified by liquid chromatography-mass spectrometry(LC-MS).The ACE inhibitory activity was verified using the synthetic oligopeptides.The absorption and metabolism characteristics of the oligopeptides were predicted,a three-dimensional quantitative structure-activity relationship(3D-QSAR)model was established,and molecular docking analysis was carried out.The peptide fraction with molecular mass less than 3 kDa had the strongest ACE inhibitory effect.After further purification,nine ACE inhibitory peptides were identified from peak 1.These peptides could not interfere with the normal physiological activities of the human body.The 3D-QSAR model for LFRAF was successfully established using the comparative molecular force field analysis(CoMFA)method.The positively charged amino acid residues and the introduced large groups on the side chain at the C-terminus of the ACE inhibitory peptides could improve their activity.LFRAF occupied the S2 and S1’active pockets of the ACE peptides and bund to Zn2+to inhibit ACE activity.These results indicate that it is feasible to use subcritical water technology to degrade high-temperature sesame meal proteins to prepare ACE inhibitory peptides.
作者
孙强
王瑞丹
黄纪念
芦鑫
宋国辉
游静
SUN Qiang;WANG Ruidan;HUANG Jinian;LU Xin;SONG Guohui;YOU Jing(Research Center of Agricultural Products Processing,Henan Academy of Agriculture Sciences,Zhengzhou 450002,China;Henan Key Laboratory of Specific Oilseed Crops Genomics,Zhengzhou 450002,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2023年第16期106-112,共7页
Food Science
基金
财政部和农业农村部:国家现代农业产业技术体系建设专项(CARS-14)
河南省农业科学院科技创新团队项目(2022TD11)。
关键词
芝麻蛋白
血管紧张素转化酶抑制肽
分离纯化
构效关系
安全性评价
sesame protein
angiotension converting enzyme inhibitory peptide
isolation and purification
structure-activity relationship
safety evaluation
