摘要
目的观察创伤性脑损伤(TBI)大鼠脑组织中胶质纤维酸性蛋白(GFAP)的表达及脑区之间的差异变化。方法采用改良Feeney自由落体法制备大鼠TBI模型,将其随机分为假手术组(Sham组)和模型组(TBI组)。采用免疫组化染色、Western blot和RT-qPCR实验方法,观察TBI后不同脑区活化星形胶质细胞的形态学变化以及GFAP表达情况。结果免疫组化染色结果显示,TBI右侧各脑区的星形胶质细胞均发生激活,并且波及到对侧(左侧)的脑区仅有皮质1区和中脑区。RT-qPCR结果显示,TBI右侧各脑区GFAP的mRNA均出现高表达,并波及到对侧的皮质和中脑区。Western blot结果显示,TBI右侧各脑区GFAP蛋白均出现高表达,并波及到对侧的中脑区。结论TBI会引起右侧各脑区的星形胶质细胞激活以及GFAP表达增多,同时会累及对侧(左侧)的皮质1区和中脑区。
Objective To observe the expression of glial fibrillary acidic protein(GFAP)in the brain tissue of rats with traumatic brain injury(TBI)and the differential changes between brain regions.Methods The TBI model of rats was prepared by the modified Feeney free fall method,and it was randomly divided into the sham-operated group(Sham group)and the model group(TBI group).Immunohistochemical staining,Western blot and RT-qPCR were used to observe the morphological changes of activated astrocytes and the expression of GFAP in different brain regions after TBI.Results Immunohistochemical staining showed that astrocytes were activated in all brain regions on the right side of TBI,and the only brain regions that spilled over to the contralateral side(left)were cortical region 1 and midbrain regions.RT-qPCR results showed that GFAP mRNA was highly expressed in all brain regions on the right side of TBI,affecting the contralateral cortex and midbrain regions.Western blot results showed that GFAP protein was highly expressed in all brain regions on the right side of TBI,and it spread to the contralateral midbrain regions.Conclusion TBI can cause the activation of astrocytes and increase GFAP expression in the right brain regions,while also affecting the contralateral(left)cortex 1 and midbrain regions.
作者
曹艳
李恒希
李佳丽
凌腾晗
尹爱平
周莹
杨莉
吴海鹰
李坪
Cao Yan;Li Hengxi;Li Jiali;Ling Tenghan;Yin Aiping;Zhou Ying;Yang Li;Wu Haiying;Li Ping(Dept of Human Anatomy and Histology&Embryology,Kunming Medical University,Kunming 650500;Science and Technology Achievement Incubation Center,Kunming Medical University,Kunming 650500;Dept of Emergency,The First Affiliated Hospital of Kunming Medical University,Kunming 650032)
出处
《安徽医科大学学报》
CAS
北大核心
2023年第11期1828-1832,1840,共6页
Acta Universitatis Medicinalis Anhui
基金
国家自然科学基金(编号:82060241)
云南省科技厅科技计划项目(编号:202101AY070001-002)。
