摘要
目的:研究2-甲氧基肉桂醛(2-MCA)对大鼠血管平滑肌细胞(VSMCs)增殖、迁移及表型转化的影响。方法:从大鼠主动脉中提取原代VSMCs体外培养,用10 ng/mL的血小板衍生生长因子-BB(PDGF-BB)和不同浓度2-MCA处理细胞24 h,将其分为对照组、PDGF-BB组和PDGF-BB+2-MCA组。采用CCK8法检测药物毒性及细胞增殖情况,划痕实验及Transwell小室检测细胞迁移情况,Western blot法检测SMMHC、SMTN、OPN蛋白表达情况。结果:与对照组比较,PDGF-BB能够明显促进VSMCs增殖(P<0.0001)和迁移(P<0.01),并且使OPN表达增多,SMMHC和SMTN表达减少(P<0.05);2-MCA干预后,VSMCs增殖和迁移均减少(P<0.05),SMMHC和SMTN表达增多,OPN表达减少(P<0.05)。结论:2-MCA能够抑制PDGF-BB诱导的VSMCs表型转化,进而抑制其增殖和迁移。
Objective:To investigate the effects of 2-methoxycinnamicaldehyde(2-MCA)on the proliferation,migration,and phenotype transformation of rat vascular smooth muscle cells(VSMCs).Method:Primary VSMCs were extracted from rat aorta and cultured in vitro.Cells were treated with 10 ng/mL platelet derived growth factor BB(PDGF BB)and different concentrations of 2-MCA for 24 hours.The cells were divided into control group,PDGF BB group,and PDGF BB+2-MCA group.The CCK8 method was used to detect drug toxicity and cell proliferation,scratch test and Transwell chamber were used to detect cell migration,and Western blot was used to detect the expression of SMMHC,SMTN,and OPN proteins.Result:Compared with the control group,PDGF-BB significantly promoted VSMCs proliferation(P<0.0001)and migration(P<0.01),and increased OPN expression,while decreased SMMHC and SMTN expression(P<0.05).After 2-MCA intervention,the proliferation and migration of VSMCs decreased(P<0.05),and the expression of SMMHC and SMTN increased,while the expression of OPN decreased(P<0.05).Conclusion:2-MCA can inhibit the phenotype transformation of VSMCs induced by PDGF-BB,thereby inhibiting their proliferation and migration.
作者
李慧歆
粟裕冬
董波
LI Huixin;SU Yudong;DONG Bo(Shandong University of Traditional Chinese Medicine,Jinan 250014,China;Shandong Provincial Hospital Affiliated to Shandong First Medical University,Jinan 250021,China)
出处
《中医药信息》
2023年第11期1-6,共6页
Information on Traditional Chinese Medicine
基金
国家自然科学基金项目(81870283)
泰山学者(ts20190979)。
关键词
2-甲氧基肉桂醛
血管平滑肌细胞
表型转化
2-Methoxycinnamaldehyde
Vascular smooth muscle cells
Phenotype transformation
