摘要
目的研究白及的核型、基因组大小等特征信息。方法双色荧光原位杂交分析白及的染色体核型;以番茄为内参,流式细胞术检测白及基因组大小;基因组Survey分析获得白及基因组大小、杂合率、GC含量等生物学信息。结果DAPI荧光染色及端粒荧光原位杂交发现白及染色体为二倍体,染色体类型为sm和st型,核型类型为2C。rDNA荧光原位杂交发现白及染色体有1对显示较强的5S rDNA位点和1对18S rDNA位点,5S rDNA位点位于2个同源染色体间隙,18S rDNA位点位于染色体短臂的次缢痕部位。白及基因组大小为2.37 Gb,同时全基因Survey分析得到有效数据60.11 Gb,经过K-mer分析修正及Genomescope评估,白及基因组大小约为2.53 Gb,杂合率约为1.099%,重复序列约占67.45%,GC含量为36.11%,总测序深度为23.73。结论本研究首次获得白及基于5S rDNA和18S rDNA荧光原位杂交核型及增补了白及属植物的基因组大小数据,为白及后续的物种分类、种群进化研究及全基因组测序、组装及去冗余处理等工作提供基础参考数据。
AIM To study the karyotype,genome size and other characteristics information of Bletilla striata(Thumb.)Reich b.f..METHODS B.striata had its karyotype analyzed by two-color fluorescent in situ hybridization(FISH);its genome size estimated by flow cytometry,with tomato as an internal reference;its biological information about genomic size,heterozygosity rate,GC content,etc.by genomic survey analysis.RESULTS DAPI fluorescence staining and FISH of telomere revealed that B.striata sm and st diploid chromosomes possessing karyotype 2C,with one pair strongly displaying 5S rDNA sites in the gap between two homologous chromosomes,and one pair of 18S rDNA sites in the secondary constriction site of the short arm of the chromosome analyzed by RDNA FISH.After K-mer analysis correction and Genoscope evaluation,B.striata’s 2.37 Gb genome size and 60.11 Gb effective data revealed by whole gene survey analysis turned out to be 2.53 Gb genome size,coexisting with 1.099%heterozygous rate,67.45%repetitive sequences,36.11%GC content,and 23.73 total sequencing depth.CONCLUSION Through this study,B.striata had its karyotype determined based on 5S rDNA and 18S rDNA FISH,and its genome size data supplemented for the first time,providing basic reference data for species classification,population evolution research,whole genome sequencing,assembly and redundancy processing of B.striata.
作者
杨渊
黄明进
王大昌
阮宝丽
杨秋悦
杨洋
罗影子
覃玉强
YANG Yuan;HUANG Ming-jin;WANG Da-chang;RUAN Bao-li;YANG Qiu-yue;YANG Yang;LUO Ying-zi;QIN Yu-qiang(College of Agriculture,Guizhou University,The Dendrobium Research Institute of Guizhou University,Guizhou Key Laboratory of Medicinal Plant Breeding and Planting,Guiyang 550025,China;Guizhou Chuangheyuan Agricultural Technology Co.,Ltd.,Guiyang 550025,China)
出处
《中成药》
CAS
CSCD
北大核心
2023年第11期3677-3682,共6页
Chinese Traditional Patent Medicine
基金
贵州省科学技术厅项目(黔科合支撑[2021]246号)
贵州创禾源中药产业创新发展项目(创禾源科技合同[2021]001号)。
关键词
白及
基因组调查
流式细胞术
K-mer分析
双色荧光原位杂交
Bletilla striata(Thumb.)Reich b.f.
genomic investigation
flow cytometry
K-mer analysis
two-color fluorescence in situ hybridization
