摘要
目的:对药用植物何首乌内生真菌分离、纯化;并对所得的各类菌落进行分子鉴定。方法:通过组织培养药用植物何首乌的内生真菌并对其进行分离纯化,通过外观和显微镜对药用植物何首乌内生真菌分离所得的菌株进行形态鉴定,应用PCR技术对分离菌rDNA的内转录间隔区(ITS)基因测序和核糖体大亚基(LSU)基因测序,最后对药用植物何首乌分离所得的各种内生真菌菌株进行生物学鉴定。结果:通过实验鉴定共从植物何首乌中分得7科、9属、16种内生真菌。
Objective:To isolate and purify the endophytic fungi of the medicinal plant polygonum multiflorum,and to carry out molecular identification of the obtained colonies.Methods:The endophytic fungi of the medicinal plant polygonum multiflorum were tissue cultured and isolated and purified.The strains isolated from the endophytic fungi of the medicinal plant polygonum multiflorum were morphologically identified by appearance and microscopic Transcriptional spacer(ITS)gene sequencing and ribosomal large subunit(LSU)gene sequencing,and finally biological identification of various endophytic fungal strains isolated from the medicinal plant Polygonum multiflorum.Results:A total of 7 families,9 genera and 16 endophytic fungi were identified from the plant polygonum multiflorum.
作者
杨玉竹
樊玲凤
段玉
龙艳宁
邹树良
张亚洲
Yang Yuzhu;Fan Lingfeng;Duan Yu;Long Yanning;Zou Shuliang;Zhang Yazhou(Heshouwu Research Center,Graduate School,Guizhou University of Traditional Chinese Medicine,Guiyang 550025,China;College of Food and Pharmaceutical Engineering,Guizhou Institute of Technology,Guiyang 550003,China)
出处
《山东化工》
CAS
2023年第19期77-81,共5页
Shandong Chemical Industry
基金
贵州中医药大学博士启动项目([2019]59号)
贵州理工学院科技专项项目(KJZX19-005)
贵州省科技厅项目(黔科合[2018]1071)。
关键词
何首乌
内生真菌
分离纯化
检测方法
鉴定
polygonum multiflorum
endophytic fungi
isolation and purification
detection method
identification
