摘要
为探讨小麦胚芽作为黄颡鱼(Tachysurus fulvidraco)雌性亲鱼培育饲料原料的可行性,研究以黄颡鱼雌性亲鱼(825尾)为实验对象,通过投喂不同小麦胚芽添加量0、5%、10%、15%和20%(w/w)饲料的养殖实验,比较研究了黄颡鱼雌性亲鱼的肝脏抗氧化能力、血浆生理生化指标、成活率和繁殖性能等。结果显示,小麦胚芽能显著提高黄颡鱼雌性亲鱼肝脏抗氧化酶活性(P<0.05),小麦胚芽添加量为10%时的超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和总抗氧化能力(T-AOC)活性最高,丙二醛含量(MDA)最低,多项式曲线方程显示添加量在11.57%—14.0%抗氧化性能最强。当小麦胚芽添加量达到10%时,对血浆葡萄糖(GLU)含量没有影响(P>0.05),高密度脂蛋白胆固醇(HDL-C)含量显著升高(P<0.05),低/高密度脂蛋白胆固醇含量比值(LDLC/HDL-C)显著下降(P<0.05),利于血脂的健康代谢。因此,经添加小麦胚芽的饲料进行产前和产后培育,小麦胚芽组能显著提高黄颡鱼雌性亲鱼的成活率(P<0.05),绝对繁殖力和相对体重繁殖力与对照W0组相比有所提升(P>0.05);产后培育阶段,小麦胚芽添加组W5、W10和W15畸形率显著低于对照组(P<0.05),孵化率也略高于对照组(P>0.05)。综上,小麦胚芽能够作为黄颡鱼雌性亲鱼的饲料原料,建议产前培育添加量为饲料质量的10%—15%,产后再培育添加量为饲料质量的5%—10%,能有效改善体质,提高雌性亲鱼重复使用率。
The sustainable development of the aquaculture industry for yellow catfish(Tachysurus fulvidraco)in China has been hindered by a shortage of females and inadequate scientific feeding,as well as the shortage of raw aquatic feed materials.Studies have shown that wheat germ,which containes plant functional nutrient with antioxidant,anticancer,hypolipidemic,and gonad-development promoting effects,could be a potential feed material.This study evaluated the effects of wheat germ on the survival rate,liver antioxidant capacity,plasma physiology and biochemistry,and reproductive performance of 825 female broodstock of yellow catfish,and determined the feasibility of wheat germ as feed material by feeding different wheat germ addition 0,5%,10%,15%and 20%(w/w).The results showed that wheat germ could significantly improve survival rate and liver antioxidant capacity of the female broodstock(P<0.05).Wheat germ significantly improved the enzyme activity of the female broodstock(P<0.05).The activity of superoxide dismutase(SOD),catalase(CAT),and total antioxidant capacity(T-AOC)enzyme was the highest in the 10%wheat germ addition diet,with which the content of malondialdehyde(MDA)was the lowest.The polynomial curve equation showed that the wheat germ addition of 11.57%—14.0%in the diet had the highest antioxidant activity.When the addition amount of wheat germ reached 10%,it did not significantly affect plasma glucose(GLU)(P>0.05).It caused a significant increase in the content of high-density lipoprotein cholesterol(HDL-C)(P<0.05),and a significant decrease in the ratio of low-density lipoprotein cholesterol to high-density lipoprotein cholesterol(LDL-C/HDL-C)(P<0.05).In conclusion,wheat germ can be a beneficial feed material for female parents of yellow catfish,particularly during the pre-spawning period with an addition amount of 10%—15%of diet quality and during the post-spawning period with an addition amount of 5%—10%.The present study provides an important foundation for further broodstock feed development and cultivation.
作者
李亚宁
陈敏
刘洋
韩磊
何焱
樊启学
沈志刚
LI Ya-Ning;CHEN Min;LIU Yang;HAN Lei;HE Yan;FAN Qi-Xue;SHEN Zhi-Gang(Key Laboratory of Freshwater Animal Breeding,Ministry of Agriculture,Engineering Research Center of Green development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt,Ministry of Education,Hubei Provincial Engineering Laboratory for Pond Aquaculture,College of Fisheries,Huazhong Agricultural University,Wuhan 430070,China;Hubei Huangyouyuan Fishery Development Limited Company,Wuhan 430070,China)
出处
《水生生物学报》
CAS
CSCD
北大核心
2024年第2期264-274,共11页
Acta Hydrobiologica Sinica
基金
国家自然科学基金面上项目(32273122)资助。
关键词
小麦胚芽
雌性亲鱼
繁殖力
成活率
抗氧化性能
生理生化指标
黄颡鱼
Wheat germ
Female broodstock
Fecundity
Survival rate
Oxidation resistance
Physiological and biochemical indexes
Tachysurus fulvidraco
