摘要
目的从肠道菌群角度分析优化溃结方治疗溃疡性结肠炎(UC)的基本原理。方法采用三硝基苯磺酸(TNBS)/乙醇二次致炎法结合束缚法制备UC大鼠模型。将24只SD大鼠随机分为正常组、模型组、优化溃结方组、柳氮磺胺吡啶组,每组6只。分析4组大鼠治疗前后结肠炎疾病活动指数(DAI),粪便标本菌群的变化。结果与模型组比较,优化溃结方组、柳氮磺胺吡啶组DAI均差异有显著性(P<0.05)。柳氮磺胺吡啶组与优化溃结方组DAI评分比较,无差异(P>0.05)。正常组与模型组存在显著差异的菌属,排名前9位的属水平OTU分别为粪杆菌(Faecalibacterium)、经黏液真杆菌属(Blautia)、狭义梭菌属1(Clostridium sensu stricto 1)、异杆菌属(Allobaculum)、拟杆菌(Bacteroides)、Ruminococcaceae.UCG.013、苏黎世杆菌属(Turicibacter)、乳酸菌(Lactobacillus)、罗姆布茨菌(Romboutsia)。给予优化溃结方治疗后,厚壁菌门、芽孢杆菌纲、乳杆菌目、乳杆菌科、双歧杆菌科、格氏乳杆菌种、鼠乳杆菌种、乳酸菌属相对丰度显著增加(P<0.01);拟杆菌门、变形菌门、梭状芽孢杆菌纲、拟杆菌纲、丹毒丝菌纲、γ变形菌纲、梭菌目、丹毒丝菌、拟杆菌属、Turicibacter、狭义梭菌属1、Ruminococcaceae UCG-013相对丰度显著减少(P<0.01)。优化溃结方组治疗后罗姆布茨菌属、异杆菌属、苏黎世杆菌属多于柳氮磺胺吡啶阳性药组。结论优化溃结方通过增加UC气滞血瘀模型SD大鼠肠道有益菌、减少有害菌的相对丰度,改变肠道细菌数量,降低DAI,降低肠道炎性反应,从而治疗疾病。
Objective To analyze the mechanism of the optimized ulcerative colitis(UC)decoction in the treatment of UC from the perspective of gut microbiota.Methods A UC rat model was prepared using trinitrobenzene sulfonic acid(TNBS)/ethanol secondary inflammation method combined with the restraint method.A total of 24 Sprague-Dawley(SD)rats were randomly divided into the normal group,model group,optimized UC decoction group,and sulfasalazine(SSZ)group,with 6 rats in each group.Fecal specimens were collected.Changes in the disease activity index(DAI)for UC and fecal microbiota in UC rats with Qi stagnation and blood stasis before and after treatment were analyzed.Results There was a significant difference in the DAI for UC in the optimized UC decoction group and SSZ group compared with that of model group(P<0.05),which was comparable between the former two groups(P>0.05).There were significant differences in the abundances of fecal microbiota between the normal group and the model group.The top 9 differentially expressed gut microbiota(relative OTU composition)at the genus level were the Faecalibacterium,Blautia,Clostridium sensu stricto 1,Allobaculum,Bacteroides,Ruminococcaceae.UCG.013,Turicibacter,Lactobacillus,and Romboutsia.After the treatment of the optimized UC decoction,relative abundances of the Firmicutes,Bacillibacteria,Lactobacillales,Lactobacilliaceae,Bifidobacteriaceae,Lactobacillus gasseri species,Lactobacillus murine species,and Lactobacillus species significantly increased(P<0.01),while those of Bacteroidetes,Proteobacteria,Clostridia,Bacteroidetes,Erysipelothrix,Gammaproteobacteria,Clostridiales,Erysipelothrix,Bacteroidetes,Turicibacter,Clostridium sensu stricto 1 and Ruminococcaceae UCG.013 significantly decreased(P<0.01).After the treatment of the optimized UC decoction,relative abundances of the Rhombusia species,Allobacterium species,and Zurichella species were significantly higher than those of SSZ group.Conclusion The optimized UC decoction lowers inflammatory factor levels in UC rats with Qi stagnation and blood stasis via increasing the relative abundances of beneficial bacteria and decreasing those of harmful bacteria.It reduces DAI for UC and alleviates intestinal inflammatory response by changing the abundances of intestinal bacteria,thereby achieving the therapeutic purpose.
作者
李娜
沈江立
柳越冬
吴宪树
王磊
盛天骄
姚红娟
LI Na;SHEN Jiangli;LIU Yuedong(Xianyang Central Hospital,Shaanxi,Xianyang 712000,China;不详)
出处
《河北医药》
CAS
2023年第23期3529-3534,共6页
Hebei Medical Journal
基金
陕西省中医药管理局秦创原中药创新研发项目(编号:2022-QCYZH-015)
陕西省自然科学基础研究计划项目(编号:2022JM-489)。
关键词
溃疡性结肠炎
优化溃结方
肠道菌群
炎症因子
ulcerative colitis
optimized ulcerative colitis(UC)decoction
gut microbiota
inflammatory factors
