摘要
间接免疫荧光法是检测抗核抗体的参考方法,然而检测过程标准化程度低、检测结果报告一致性差是临床实验室面临的主要挑战。随着抗核抗体荧光模型国际共识组织对各荧光模型进行标准化命名,以人喉癌上皮细胞为基质的间接免疫荧光法实验由手工操作逐步转向全自动仪器操作以及计算机辅助诊断系统的临床应用,实验室对该项目的规范化检测及结果报告提出了新的要求。本共识从检验项目开展前、检验过程和结果报告3个方面进行阐述并提出相应意见,旨在进一步推进该项目在我国实验室检测与报告的规范化与标准化。
The indirect immunofluorescence assay(IFA)is a reference method for detecting antinuclear antibodies(ANA).However,the insufficient standardization of the detection process and the poor consistency of test result reporting are the main challenges faced by clinical laboratories.With the release of ANA patterns nomenclature by the International Consensus on ANA Patterns,the gradual shifting from manual operations to automatic ANA detecting devices and the clinical application of computer-aided diagnosis systems,the clinical laboratories are facing new challenges on the standardized detection and accurate test report.This consensus elaborates and puts forward corresponding opinions of three aspects including before IFA test implementing,examination phase and results report in order to further promote the normalization and standardization in testing and reporting ANA by HEp-2 IFA in China.
出处
《中华检验医学杂志》
CAS
CSCD
北大核心
2023年第11期1149-1163,共15页
Chinese Journal of Laboratory Medicine
基金
国家重点研发计划(2022YFC2603800)。
关键词
荧光抗体技术
间接
抗体
抗核
临床实验室技术
专家共识
Fluorescent antibody technique,indirect
Antibodies,anti-nuclear
Clinical laboratory techniques
Consensus
