摘要
目的:研究广东地区尘螨过敏儿童脂多糖受体基因(CD14)多态性和等位基因频率的分布情况,探讨其多态性与儿童尘螨过敏及与血浆IgE水平的关系。方法:对161例尘螨过敏患儿(尘螨过敏组)、186例疾病对照组和100名健康儿童(正常对照组)用散射比浊法测定血浆总IgE水平,采用酶联免疫捕获法进行过敏原特异性IgE(sIgE)检测,采用聚合酶链反应一限制性片段长度多态性(PCR-RFLP)法进行基因型分析,调查多态位点的分布特征,比较不同基因型的血浆尘螨特异性IgE水平,比较3组儿童多态位点的基因差异。结果:3组儿童均发现CDl4-159T/C存在不同的基因型,均以CC、CT和TT基因型多见,尘螨过敏组频率分别为55.9%、33.5%和10.6%,疾病对照组频率分别为10.2%、34.9%和54.8%,正常对照组分别为35%、47%和18%。尘螨过敏组、疾病对照组和正常对照组间基因型差异具有统计学意义(P<0.05),疾病对照组与正常对照组间差异有统计学意义(P<0.05),其他组间差异无统计学意义。与正常对照组相比,尘螨过敏组的CD14 CC基因型携带频率增高,TT基因型携带频率较低。疾病对照组同组内T等位基因携带者IgE水平高于非携带者(P<0.05);同一基因型中,尘螨过敏组和疾病对照组IgE水平高于正常对照组(P=0.02)。结论:广东地区儿童尘螨过敏患儿存在CD14基因启动子C-159T多态性,C等位基因与血浆IgE升高相关。
Objective:To study the distribution of lipopolysaccharide receptor gene(CD14)polymorphism and allele frequency in dust mite allergic Han children in Guangdong,and to explore the relationship between CD14 polymorphism and dust mite allergy in children and plasma IgE level.Methods:The case group was selected according to the following criteria:(1)Han children aged 2~14 years;(2)The clinical diagnosis was asthma,allergic rhinitis or atopic dermatitis;(3)Total serum IgE>200IU/mL:(4)The serum specific IgE of dust mite was positive.A total of 161 cases of dust mite allergy,186 cases of disease control group and 100 healthy children(normal control group)were tested for total IgE level in the plasma by nephelometry,and allergen specific IgE(slgE)was detected by enzyme-linked immunocapture method.Polymerase chain reaction restriction fragment length polymorphism(PCR-RFLP)was used to analyze the genotypes,investigate the distribution of polymorphic loci,compare the specific IgE levels of dust mites in different genotypes,and compare the genetic characteristics of polymorphic loci between the two groups of children.Results:Single nucleotide polymorphisms(SNPs)of cdl4-159t/c were found in all three groups.CC,CT and TT genotypes were the most common.Their frequencies in dust mite allergy group were 55.9%,33.5%and 10.6%;10.2%,34.9%and 54.8%in disease control group;35%,47%and 18%in normal control group,respectively.There was significant difference in genotype among dust mite allergy group,disease control group and normal control group(P<0.05).Compared with the normal control group,the frequency of CD14CC genotype was higher and that of TT genotype was lower in the dust mite allergic group.In the control group,the plasma IgE level in T allele cariers was higher than that in non-carriers(P<0.05).In the same genotype,the IgeE level in the case group was higher than that in the control group(P-0.02).Conclusion:Cdl4 gene promoter C-159T polymorphism exists in Han children with dust mite allergy in Guangdong,and the C allele is associated with the increase of plasma IgE.
作者
李丰
陈慧珊
曾华松
LI Feng;CHEN Huishan;ZENG Huasong(Department of allergy,immunity and rheumatism,Guangzhou Children's Hospital,Guangzhou Women's and children's Medical Center,Guangzhou 510120,China)
出处
《临床皮肤科杂志》
CAS
CSCD
北大核心
2023年第12期712-714,共3页
Journal of Clinical Dermatology
