摘要
芦苇(Phragmites australis)分布范围广,生物量巨大,环境适应性强。受芦苇多倍体基因组的限制,在基因水平上解释其独特的物种特质非常困难。利用三代测序及多种方法研究2种生态型芦苇(2n=8x)中RCA基因(编码Rubisco活化酶)的类型、结构、表达和定位模式。结果表明,芦苇基因组中有4类RCA基因,均属于RCA2β。转录水平和蛋白质水平检测以及免疫胶体金定位结果表明,芦苇RCA的基因表达模式和蛋白质定位具有明显的生态型特异性。与沼泽芦苇(SR)相比,沙丘芦苇(DR)中RCA的表达水平较低,且更倾向于产生RCA2-β2产物。通过分相的蛋白双向电泳和质谱方法,鉴定到6个高表达的RCA同型物。在DR中这些同型物高比例地分布于膜相。研究表明当芦苇处于极度恶劣的沙漠环境中, RCA转移到膜相并参与叶绿体的膜保护机制。
Phragmites australis(reed)is a cosmopolitan species,with huge biomass and high environmental adaptability.Restricted by its polyploidy genome,it is very difficult to study its unique properties at the genetic level.Using third-generation sequencing and multiple methods,we comprehensively studied the type,structure,expression,and localization patterns of RCA genes(encoding Rubisco activating enzyme)in two reed ecotypes(2n=8x).There are four types of RCA genes in Phragmites genome,all belonging to the RCA2βcategory.At the transcriptional and protein levels,we adopted immunogold method to detect the localization of RCA proteins.We found that RCAs have obvious ecotype specificity.Compared with swamp reeds(SR),desert-dune reeds(DR)have low RCA expression and RCA2-β2 are preferentially expressed.Six highly expressed RCA isoforms were identified though two-dimensional electrophoresis(2-DE)and mass spectrometry(MS),and were distributed in a high proportion in membrane fraction in DR.This result supports the hypothesis that RCA may transfer to the membrane area as the environment deteriorates,thereby giving RCA a protective function on the chloroplast membrane.
作者
裘天航
王安安
李利
王迎春
崔继鹏
王紫瑶
王蕊
崔素霞
Tianhang Qiu;An’an Wang;Li Li;Yingchun Wang;Jipeng Cui;Ziyao Wang;Rui Wang;Suxia Cui(Beijing Key Laboratory of Plant Gene Resources and Biotechnology for Carbon Reduction and Environmental Improvement,College of Life Sciences,Capital Normal University,Beijing 100048,China)
出处
《植物学报》
CAS
CSCD
北大核心
2023年第5期687-700,共14页
Chinese Bulletin of Botany
基金
国家自然科学基金(No.31972934)。
关键词
芦苇
八倍体
沙漠生态型
RCA
膜相定位
Phragmites australis
octoploid
desert ecotype
RCA
membrane localization
