摘要
目的观察淫羊藿苷对人前列腺癌PC3细胞增殖及凋亡的影响,并探讨可能机制。方法取对数期PC3细胞,随机分为对照组、淫羊藿苷组、激动剂组、联合组。对照组常规培养,淫羊藿苷组加入淫羊藿苷(100μmol/L),激动剂组加入SB216763(20μmol/L),联合组加入淫羊藿苷(100μmol/L)与SB216763(20μmol/L)。MTT法检测细胞增殖能力;流式细胞术检测细胞凋亡率;ELISA法检测细胞上清液血管内皮生长因子(VEGF)水平;免疫印迹法检测细胞糖原合成酶激酶3β(glycogen synthase kinase 3β)、β-连环蛋白(β-catenin)表达。结果与对照组比较,淫羊藿苷组24、48、72 h吸光度值降低,上清液VEGF水平及β-catenin蛋白表达降低,凋亡率、GSK-3β蛋白表达升高(P<0.05);激动剂组24、48、72 h吸光度值升高,上清液VEGF水平及β-catenin蛋白表达升高,凋亡率、GSK-3β蛋白表达降低(P<0.05);与淫羊藿苷组比较,联合组24、48、72 h吸光度值升高,上清液VEGF水平及β-catenin蛋白表达升高,凋亡率、GSK-3β蛋白表达降低(P<0.05);与激动剂组比较,联合组24、48、72 h吸光度值降低,上清液VEGF水平及β-catenin蛋白表达降低,凋亡率、GSK-3β蛋白表达升高(P<0.05)。结论淫羊藿苷可抑制前列腺癌细胞增殖及血管新生,促进其凋亡,其作用机制可能与抑制Wnt/β-catenin信号通路活性有关。
Objective To observe the effect of icariin on the proliferation and apoptosis of human prostate cancer PC3 cells,and to explore its possible mechanism.Methods PC3 cells in log phase were taken and randomly divided into the control group,icariin group,agonist group and combination group.The control group was routinely cultured,the icariin group was added with icariin(100μmol/L),the agonist group was added with SB216763(20μmol/L),and the combination group was added with icariin(100μmol/L)and SB216763(20μmol/L).The cell proliferation was detected by MTT assay.The apoptosis rate was detected by flow cytometry.The level of vascular endothelial growth factor(VEGF)in the cell supernatant was detected by ELISA.The protein expressions of glycogen synthase kinase 3β(glycogen synthase kinase 3β)andβ-catenin were detected by western blotting.Results Compared with the control group,the absorbance values at 24,48 and 72 h,the level of VEGF in the supernatant and the expression ofβ-catenin protein were decreased,while the apoptosis rate and the expression of GSK-3βprotein were increased in the icariin group(P<0.05),the absorbance values at 24,48,and 72 h,the level of VEGF in the supernatant and the expression ofβ-catenin protein were increased,while the apoptosis rate and the expression of GSK-3βprotein were decreased in the agonist group(P<0.05).Compared with the icariin group,the absorbance values at 24,48 and 72 h,the level of VEGF in the supernatant and the expression ofβ-catenin protein were increased,while the apoptosis rate and the expression of GSK-3βprotein were decreased in the combined group(P<0.05).Compared with the agonist group,the absorbance values at 24,48,and 72 h,the level of VEGF in the supernatant and the expression ofβ-catenin protein were decreased,while the apoptosis rate and the expression of GSK-3βprotein were increased in the combined group(P<0.05).Conclusion Icariin can inhibit the proliferation and angiogenesis of prostate cancer cells and promote its apoptosis,and its mechanism may be related to inhibit the Wnt/β-catenin signaling pathway activity.
作者
李磊
武海波
邢伟只
LI Lei;WU Haibo;XING Weizhi(The Third People's Hospital of Henan,Zhengzhou,450006)
出处
《实用癌症杂志》
2023年第12期1935-1939,共5页
The Practical Journal of Cancer
