摘要
目的 建立金花茶花的高效液相(HPLC)指纹图谱,对其化学成分进行鉴定,并考察其体外抗氧化能力。方法70%乙醇溶液回流法提取10批次金花茶花,采用Agilent Eclipse Plus C18(250 mm×4.6 mm,5μm)色谱柱,梯度洗脱,建立金花茶花指纹图谱,应用HPLC-MS法分析其化学成分,并用1,1-二苯基-2-苦基肼自由基(DPPH)和2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐(ABTS)法评价金花茶花的抗氧化能力。结果 建立金花茶花共有HPLC色谱图,标定共有峰11个,经过中药色谱指纹图谱相似度评价软件(2012版)计算,相似度均在0.98以上。HPLC-MS分析结果显示,金花茶花中成分多为黄酮类化合物。抗氧化能力检测结果显示,金花茶花对DPPH自由基和ABTS自由基均具有较好的清除能力,并呈浓度相关性。结论 建立了金花茶花药材的指纹图谱,其含有丰富的黄酮类化合物,具有良好的体外抗氧化作用。
Objective To establish a HPLC fingerprint of Camellia nitidissima Chi,analyze its chemical components,and study its antioxidant capacity.Methods Extraction of ten batches of Camellia nitidissima Chi were made by refluxing with 70%ethanol solution.Agilent Eclipse Plus C18(250 mm×4.6 mm,5μm)Chromatographic column were used,gradient elution,to establish a fingerprint of Camellia nitidissima Chi,analysis of its chemical components using HPLC-MS,and evaluation of the antioxidant capacity of Camellia nitidissima Chi using DPPH and ABTS methods.Results The HPLC chromatogram of Camellia nitidissima Chi was established,and 11 peaks were calibrated.The similarity was all above 0.98 through calculation of TCM chromatographic fingerprint similarity evaluation software(2012 edition).Meanwhile,HPLC-MS analysis showed that the components of Camellia nitidissima Chi were mostly flavonoids,and the antioxidant capacity of Camellia nitidissima Chi was tested.The results showed that it had good antioxidant capacity to DPPPH free radicals and ABTS free radicals in vitro,and showed a certain concentration dependence.Conclusion We had established a fingerprint of Camea nitidissima Chi,which was rich in flavonoids and had good in vitro antioxidant effects.
作者
田光卉
王志允
张彦昕
赵倩
侯文彬
TIAN Guanghui;WANG Zhiyun;ZHANG Yanxin;ZHAO Qian;HOU Wenbin(Tianjin Tasly Modern Traditional Chinese Medicine Resources Co.,LTD.,Tianjin 300410,China;Institute of Radiation Medicine,Chinese Academy of Medical Sciences and Peking Union Medical College,Tianjin 300190,China;Tianjin Nankai District Wangdingdi Hospital,Tianjin 300190,China)
出处
《药物评价研究》
CAS
2023年第11期2387-2394,共8页
Drug Evaluation Research
基金
中国医学科学院医学与健康科技创新工程重大协同创新项目资助(2021-I2M-1-042)
中央高校基本科研业务费专项资金(3332022063)。
关键词
金花茶花
指纹图谱
高效液相色谱-质谱联用
黄酮类
抗氧化
Camellia nitidissima Chi
fingerprint
high performance liquid chromatography-mass spectrometry
flavonoids
antioxidant
