摘要
目的对塔城蜱病毒5(Tacheng Tick Virus 5,TcTV-5)进行检测,为新发蜱媒传染病的监测提供参考。方法2019—2021年采集新疆维吾尔自治区(新疆)6县(市)的寄生蜱和游离蜱451只,对其分别进行形态学和分子生物学鉴定;于2021年收集新疆图木舒克市农牧民血液样本280份,运用反转录聚合酶链式反应(RT-PCR)技术,对收集到的所有样本进行TcTV-5感染阳性率的检测,构建基因进化树,分析遗传进化关系,对检测结果进行分子流行病学分析。结果经形态和分子鉴定,在新疆采集到的蜱分属于3属3种,包括璃眼蜱属的亚洲璃眼蜱、革蜱属的边缘革蜱和扇头蜱属的图兰扇头蜱;新疆蜱与人中均可检测到TcTV-5,6个县(市)的蜱阳性率为3.33%(2/60)~36.11%(26/72),图木舒克市280份人血样本的阳性率为2.50%(7/280)。结论TcTV-5在新疆蜱与人血中均可被检出;在多种蜱种(亚洲璃眼蜱、边缘革蜱、图兰扇头蜱)中均可检测到,边缘革蜱阳性率最高。
Objective To detect Tacheng tick virus 5(TcTV-5)in ticks and humans in Xinjiang Uygur Autonomous Region(Xinjiang),and provide reference for the detection of new tick-borne infectious diseases.Methods A total of 451 free ticks and parasitic ticks were collected from 6 counties in Xinjiang from 2019 to 2021.Morphological and molecular biological tests were performed to determine tick species.In 2021,a total of 280 blood samples of farmers and herdsmen in Tumushuke of Xinjiang were collected,Reverse transcription-polymerase chain reaction(RT-PCR)was used to detect TcTV-5 in collected samples,then gene phylogenetic tree was constructed,the genetic evolutionary relationship was analyzed,and the results were analyzed by molecular epidemiological method.Results According to morphological and molecular identifications,the ticks collected in Xinjiang were classified into three genera and three species,including Hyalomma asiaticum,Dermacentor marginatus and Rhipicephalus turanicus.TcTV-5 was detected in both ticks and human blood samples.The positive rates of TcTV-5 in ticks in 6 counties ranged from 3.33%(2/60)–36.11%(26/72),and the positive rates of TcTV-5 was 2.50%(7/280)in 280 human blood samples from Tumushuke.Conclusion TcTV-5 can be detected in both ticks and human blood in Xinjiang.The ticks carrying TcTV-5 included(H.asiaticum,D.marginatus and R.turanicus)and the positive rate was highest in D.marginatus.
作者
李逢时
赵姗姗
谭文波
吾热力哈孜·哈孜汗
谷新利
王素文
刘钢
王远志
Li Fengshi;Zhao Shanshan;Tan Wenbo;Oralhazi·Hazikhan;Gu Xinli;Wang Suwen;Liu Gang;Wang Yuanzhi(School of Medicine,Shihezi University,Shihezi 832003,Xinjiang,China;College of Animal Science&Tecnology,Shihezi University,Shihezi 832003,Xinjiang,China)
出处
《疾病监测》
CAS
CSCD
北大核心
2023年第11期1377-1380,共4页
Disease Surveillance
基金
国家重点研发计划项目(No.2022YFC2304000)
国家自然科学基金项目(No.82260399)
兵团重点领域科技攻关计划项目(No.2022AB014)。
关键词
塔城蜱病毒5
蜱
新疆维吾尔自治区
蜱传病
Tacheng tick virus 5
Tick
Xinjiang Uygur Autonomous Region
Tick-borne disease
