摘要
为比较紫海胆不同家系间幼体生长发育的差异,实验构建了9个紫海胆全同胞家系,比较了其受精率、孵化率、幼体存活率、变态率、开口率、幼胆生长、性腺发育和消化道微生物组成。方差分析表明,各个家系的受精率均在95%以上,无显著性差异,而其他指标在不同家系间均存在显著性差异。孵化率、早期幼体存活率、晚期幼体存活率、变态率和开口率最高的家系分别为3号(58%)、1号(83%)、4号(90%)、2号(75%)和9号(100%),最低的家系分别为6号(0%)、3号(42%)、2号(76%)、9号(6%)和5号(24.5%)。幼胆生长速率最快是3号家系为146%;最慢的是5号家系为76%。3号家系在实验结束时性腺发育明显;而其他家系性腺未见发育。3号家系的消化道微生物中拟杆菌门丰度较高,变形菌门丰度低;生长速率较慢的5号家系则与之相反。研究结果表明,紫海胆不同家系间的生产性状存在差异,具有选育改良的潜力,实验为紫海胆良种培育积累了相关数据。
In order to compare the differences of larva growth and development among different families of Heliocidaris crassispinas,9 full-sibling families of H.crassispinas were constructed.The important indicators of larval growth and development,including fertilization rate,hatching rate,larva survival rate,metamorphosis rate and initial feeding rate,were compared in the 9 families.The results showed that the fertilization rate of every fam-ily was over 95%,without no significant difference(P>0.05),but there are significant differences in other indicat-ors in 9 families(P<0.05).The highest hatching rate,early larval survival rate,late larval survival rate,meta-morphosis rate and nitial feeding rate are family No.3(58%),family No.1(83%),family No.4(90%),family No.2(75%)and family No.9(100%),respectively,and the lowest are family No.6(0%),family No.3(42%),family No.2(76%),family No.9(6%)and family No.5(24.5%),respectively.The growth rate of family No.3 is 146%,which is the fastest in all families.While,the slowest one is No.5 families(76%).At the end of the experiment,the gonad development of family No.3 was obvious;the other families were not,yet.The Proteobacteria proportion of family No.3 is high,but Bacteroidetes proportion is low.The proportion of the two phyla of family No.5 is oppos-ite to that of family No.3.The results show that there are differences in production traits among different families of H.crassispinas,which has the potential for breeding and improving,and this study accumulate relevant data for the cultivation of H.crassispinas.
作者
钟幼平
酆伟
张丽莉
黄世玉
王国栋
ZHONG Youping;FENG Wei;ZHANG Lili;HUANG Shiyu;WANG Guodong(Fisheries College,Jimei University,Xiamen 361021,China;Key Laboratory of Healthy Mariculture for the East China Sea,Ministry of Agriculture and Rural Affairs,Jimei University,Xiamen 361021,China)
出处
《水产学报》
CAS
CSCD
北大核心
2023年第12期58-70,共13页
Journal of Fisheries of China
基金
国家重点研发项目(2018YD0901605)
福建省科技厅引导性项目(2020N0014)。
关键词
紫海胆
家系
早期发育
生长
变态
Heliocidaris crassispinas
genealogy
early development
growth
metamorphosis
