摘要
目的观察参柏祛毒袋泡剂药效学和安全性指标。方法SD大鼠60只(雌30只,雄30只),体重150~170 g,按照随机数字表法将其分为空白组,参柏高(1.050 g生药/ml)、中(0.525 g生药/ml)、低(0.263 g生药/ml)剂量组,皮炎平(0.600 g/只)组,各12只。大鼠左后足跖皮下注射1%角叉菜胶0.1 ml,各组给药后测量1~5 h足跖容积,计算肿胀度。昆明小鼠48只,雌性,体重18~24 g,按照随机数字表法将其分成空白组,参柏高(1.750 g生药/ml)、中(0.875g生药/ml)、低剂量(0.438g生药/ml)组,各12只。测定小鼠基础痛阈,再以40℃温度药浴各组分别泡足20 min,测量给药后5个时间点小鼠痛阈,计算痛阈提高百分率。豚鼠30只,雌雄兼用,体重280~320 g,按照随机数字表法将其分成空白组、2,4-二硝基氯苯组、参柏组,各10只。取参柏0.2 ml持续6 h涂在右侧去毛区,第7天和第14天各重复1次,末次给药后14 d,将各组药物0.2 ml涂于豚鼠左侧去毛区,即刻和24、48、72 h观察局部皮肤及全身情况,进行评价。结果参柏高剂量组、中剂量组5 h较3 h足肿胀度低(P<0.05)。参柏高、中剂量组较空白组2~3 h足肿胀度低(P<0.05),低剂量组较空白组3 h足肿胀度低(P<0.05)。参柏高剂量组0.5~4.5 h,参柏中剂量组1.5~4.5 h,参柏低剂量组3.5~4.5 h较本组基础痛阈高(P<0.05)。参柏高剂量组0.5~4.5 h痛阈高于空白组(P<0.05),中剂量组1.5~3.5 h痛阈高于空白组(P<0.05)。参柏祛毒袋泡剂无致敏性。结论参柏祛毒袋泡剂具有明显的抗感染、镇痛作用,且药物外用安全。
Objective To observe the pharmacodynamics and safety indicators of Shenbai-Qudu Teabag.Methods A total of 60 SD rats(30 females and 30 males)with body weight of 150-170 g were divided into blank group,Shenbai high(1.050 g crude drug/ml),medium(0.525 g crude drug/ml),and low(0.263 g crude drug/ml)dose groups and Piyanping(0.600 g/ml)group according to random number table method,with 12 rats in each group.0.1 ml of 1%carrageenan was injected subcutaneously into the left posterior foot of rats,the hourly plantar volume was measured between 1-5 h after administration,and the swelling degree was calculated.Forty-eight Kunming mice,females,weight 18-24 g,were divided into blank group,Shenbai high(1.750 g crude drug/ml),medium dose(0.875 g crude drug/ml),and low dose(0.438 g crude drug/ml)groups according to random number table method,with 12 cases in each group.The basal pain threshold of mice was determined,and then the mice were soaked in a drug bath at 40℃for 20 minutes,respectively.The pain threshold of mice was measured at five time points after administration,and the percentage increase of pain threshold was calculated.A total of 30 male and female guinea pigs with body weight of 280-320 g were divided into blank group,2,4-dinitrochlorobenzene group,and ginseng and cypress group according to random number table method,with 10 guinea pigs in each group.0.2 ml of Shenbai was taken and apply it to the right depilatory area for 6 h,and repeat once on the 7th and 14th day respectively.14 days after the last administration,0.2 ml of the drug was applied to the left depilatory area of guinea pigs,and the local skin and the whole body situation immediately and 24,48,and 72 h for evaluation were observed.Results The swelling degree of foot in Shenbai high dose group and medium dose group at 5 h were lower than that at 3 h(P<0.05).The foot swelling of Shenbai high dose group and medium dose group was lower than that of blank group at 2-3 h(P<0.05),and the foot swelling of Shenbai low dose group was lower than that of blank group at 3 h(P<0.05).The basal pain threshold of Shenbai high dose group for 0.5-4.5 h,medium dose group for 1.5-4.5 h and low dose group for 3.5-4.5 h were higher than basic pain threshold of this group(P<0.05).The pain threshold of Shenbai high dose group was higher than that of blank group at 0.5~4.5 h(P<0.05),and that of medium dose group at 1.5-3.5 h was higher than that of blank group(P<0.05).Shenbai-Qudu Teabag had no sensitization effect.Conclusion Shenbai-Qudu Teabag has significant anti-inflammatory and analgesic effects when applied externally,and it is safe for topical use.
作者
贾菲
王晓锋
李国栋
王彦云
潘健
李峨
JIA Fei;WANG Xiaofeng;LI Guodong;WANG Yanyun;PAN Jian;LI E(Department of Anorectal Medicine,Guang’anmen Hospital China Academy of Chinese Medical Science,Beijing100053,China;New Drug Research and Develope Center,Guang’anmen Hospital China Academy of Chinese Medical Science,Beijing100053,China;Department of Clinical Laboratory,Guang’anmen Hospital China Academy of Chinese Medical Science,Beijing100053,China)
出处
《中国医药导报》
CAS
2023年第29期4-7,共4页
China Medical Herald
基金
国家自然科学基金面上项目(81673980)
中国中医科学院广安门医院新药基金项目(2005Y001)。
