摘要
为了发掘盐胁迫条件下紫甘薯全基因组水平基因表达的变化、关键调控网络及关联代谢途径,本研究以200 mmol·L^(-1)NaCl胁迫处理日本紫甘薯品种‘Ayamurasaki’盆栽苗0、1、6、24 h,测定盐胁迫过程中薯苗生理生化指标的变化,并利用高通量测序技术对其根系进行转录本测序和数据分析.结果表明,随着盐胁迫时间的增加,甘薯根系细胞膜受损害程度逐渐加剧.转录组结果分析显示,共检测到3202个差异表达基因,其中877个上调表达,2325个下调表达.差异表达基因KEGG路径富集分析发现,“次级代谢物生物合成”“苯丙烷生物合成”等代谢通路受胁迫影响比较大.GO分析表明,生物学过程类别中“响应胁迫”显著富集.对参与“响应胁迫”的差异基因进行表达趋势分析,并构建基因共表达网络.结果显示,活性氧清除的相关基因FeSOD、蛋白合成的相关基因P4H10、水分胁迫的相关基因PIP1-4、离子平衡与转运的相关基因LAZ1和信号转导的相关基因MEKK1等均受到显著诱导表达.共表达网络表明,NAC家族的NAC74与F-box家族的FBX23为网络核心,共同调控下游盐胁迫基因的表达.本研究可为探究甘薯的耐盐分子机制及薯类作物抗盐碱育种提供参考.
In order to obtain the transcriptomic changes and regulatory networks of purple sweetpotato under salt stress for better exploring the relative metabolic pathways,the purple sweetpotato variety"Ayamurasaki"was used to measure its changes in physiological and biochemical indexes and transcriptomes using high-throughput sequencing technique after treatment at 0,1,6 and 24 h with 200 mmol·L^(-1)NaCl.The results showed that the salt-treated plants were increasingly damaged,as evidenced by plasma membrane lipid oxidation of root,with the prolongation of salt treatment.Among 3202 identified differentially expressed genes(DEGs)by transcriptome analysis,877 genes were up-regulated and 2325 were down-regulated.Kyoto encyclopedia of genes and genomes(KEGG)pathway analysis showed that the DEGs were significantly enriched in"Biosynthesis of secondary metabolites"and"Phenylpropanoid biosynthesis".Gene ontology(GO)analysis revealed that"response to stress"was more significantly enriched in the main category of biological function.The expression trend and co-expression network of DEGs that were involved in the category of"response to stress"were analyzed and constructed.The significantly upregulated DEGs during salt treatment were identified,including reactive oxygen species scavenging gene FeSOD,protein synthetase gene P4H10,aquaporin gene PIP1-4,ion balance and transporter gene LAZ1 and signal transduction gene MEKK1.The co-expression network illustrated that NAC74 and FBX23 were the core of the network,which jointly regulate the expression of downstream genes response to salt stress.Overall,the transcriptome analysis of purple sweetpotato in response to salt stress sheds lights on understanding the molecular mechanism of salt tolerance and exploring its application in genetic improvement in root crops.
作者
鞠好庆
范维娟
孙哲
乔宇
李茹月
陈路路
陆新露
张鹏
马秋香
Ju Haoqing;Fan Weijuan;Sun Zhe;Qiao Yu;Li Ruyue;Chen Lulu;Lu Xinlu;Zhang Peng;Ma Qiuxiang(National Key Laboratory of Plant Molecular Genetics,CAS Center for Excellence in Molecular Plant Sciences,Chinese Academy of Sciences,Shanghai 200032,China;Shanghai Key Laboratory of Plant Functional Genomics&Resources,Shanghai Chenshan Botanical Garden,Shanghai 201602,China;Tai’an Academy of Agricultural Science,Tai’an 271000,Shangdong,China)
出处
《江苏师范大学学报（自然科学版）》
CAS
2023年第4期30-38,F0002,共10页
Journal of Jiangsu Normal University：Natural Science Edition
基金
中国科学院分子植物科学卓越创新中心植物性状形成与塑造重点实验室内部开放合作课题。
关键词
紫甘薯
盐胁迫
转录组
差异表达基因
调控网络
purple sweetpotato
salt stress
transcriptome
differentially expressed gene
regulatory network
