摘要
本文以日本结缕草(Zoysia japonica)为试验材料,通过喷施乙烯利后低温胁迫,检测日本结缕草叶片叶绿素、相对含水量、电解质渗透率、可溶性糖、可溶性蛋白、脯氨酸含量及脯氨酸代谢途径相关基因的表达水平,研究乙烯利对低温胁迫下结缕草生理指标、渗透调节物质及脯氨酸代谢途径相关基因的影响。结果显示,乙烯利可降低日本结缕草低温导致的叶绿素损失,抑制叶片相对含水量的下降幅度和电解质渗透率的上升幅度,提高日本结缕草可溶性糖、可溶性蛋白和脯氨酸的含量,同时上调了△1-吡咯啉-5-羧酸合成酶(P5CS)基因表达,下调了脯氨酸脱氢酶(ProDH)基因表达,对△1-吡咯琳-5-羧酸还原酶(P5CR)、△1-吡咯琳-5-羧酸脱氢酶(P5CDH)和鸟氨酸-δ-转氨酶(OAT)基因表达无影响。研究发现,乙烯利喷施处理可以缓解日本结缕草低温胁迫损伤。本研究为乙烯利对结缕草耐寒性的作用机制提供了理论基础。
This study used Zoysia japonica as an experimental material to study the influence of low temperature stress on physiological indexes,osmotic substances,and related genes of the proline metabolism pathway.To this end,we detected the expression level of chlorophyll,relative water content,electrical conductivity,soluble sugar,soluble protein,proline content,and related genes under ethephon treatment.Ethephon reduced the decrease in chlorophyll content caused by low temperature stress in Z.japonica.The fold change of relative water content and the electrical conductivity in leaves were also alleviated in ethephon-treated plants.Ethephon may regulate cell osmolytes in Z.japonica by increasing the contents of soluble sugar,soluble protein,and proline.It also upregulatedΔ1-pyrroline-5-carboxylate synthetase gene expression and downregulated proline dehydrogenase,but had no effect onΔ1-pyrroline-5-carboxylate reductase,Δ1-pyrroline-5-carboxylate dehydrogenase,and ornithine-δ-aminotransferase gene expression.In conclusion,ethylene spray treatment can alleviate low temperature stress in Z.japonica.This study provides a theoretical basis for the mechanism of action of ethephon in Z.japonica cold tolerance.
作者
李进超
张智韦
张嘉航
韩翔劲
杜洋
许立新
LI Jinchao;ZHANG Zhiwei;ZHANG Jiahang;HAN Xiangjin;DU Yang;XU Lixin(School of Grassland Science of Beijing Forestry University,Beijing 100083,China;China Coal Science and Engineering Ecological Environment Technology Co.,Ltd,Beijing 300450,China)
出处
《草业科学》
CAS
CSCD
北大核心
2023年第12期3000-3008,共9页
Pratacultural Science
基金
中央高校基本科研业务费专项资金(2021ZY83)。
关键词
叶绿素
相对含水量
电解质渗透率
可溶性糖
可溶性蛋白
脯氨酸
chlorophyll
relative water content
electrical conductivity
soluble sugar
soluble protein
proline
