摘要
针对分离筛选的一株产胞外多糖菌株TH21-44,通过形态观察、生理生化试验和16S rRNA基因系统发育分析进行种属鉴定,采用单因素和响应面法优化胞外多糖的发酵条件,并分析胞外多糖的乳化性能。结果表明,菌株TH21-44为副球菌(Paracoccus sp.);最佳产糖条件为pH7.1、接种量6.1%、温度28℃、葡萄糖7.2 g/L、酵母浸粉3.0 g/L、K_(2)HPO_(4)·3H_(2)O 0.3 g/L、MgSO_(4)·7H_(2)O 0.05 g/L及丙酮酸钠0.3 g/L,该条件下胞外多糖产量为188.89μg/mL,是优化前的3.18倍;所产胞外多糖具有良好的乳化性能,对葵花油24 h时的乳化能力为69.62%,优于吐温80。该研究为微生物胞外多糖的菌物资源开发和应用提供了依据和参考。
An exopolysaccharides(EPS)-producing strain TH21-44 was identified based on the morphological observation,physiological and biochemical tests,and 16S rRNA gene-based phylogenetic analysis.Furthermore,the fermentation conditions of the strain for the production of EPS were optimized by single-factor and response surface methods,and the emulsification performance of the EPS was evaluated.The strain TH21-44 was identified as Paracoccus sp.The optimal fermentation conditions for EPS production were pH7.1,inoculum size of 6.1%,temperature of 28℃,glucose of 7.2 g/L,yeast extract of 3.0 g/L,K_(2)HPO_(4)·3H_(2)O of 0.3 g/L,MgSO_(4)·7H_(2)O of 0.05 g/L,and sodium pyruvate of 0.3 g/L.Under these conditions,the EPS yield was 188.89μg/mL,which was 3.18 times as high as that of before optimization.The produced EPS maintained 69.62%emulsification capacity for sunflower oil after 24 h,demonstrating the emulsification performance superior to Tween 80.The findings provide the bacterial strain resources and theoretical references for the development and application of microbial exopolysaccharides.
作者
马博文
周佳
赵帅
罗宇
李海峰
屈建航
MA Bowen;ZHOU Jia;ZHAO Shuai;LUO Yu;LI Haifeng;QU Jianhang(School of Biological Engineering,Henan University of Technology,Zhengzhou 450001,Henan,China)
出处
《食品研究与开发》
CAS
北大核心
2023年第24期1-8,15,共9页
Food Research and Development
基金
国家自然科学青年基金项目(42107139)
河南工业大学创新基金支持计划专项项目(2021ZKCJ15)
郑州市R&D专项经费补助科研项目(22ZZRDZX32)。
关键词
胞外多糖
鉴定
副球菌
发酵优化
乳化
exopolysaccharides
identification
Paracoccus sp.
fermentation condition optimization
emulsification
