摘要
为促进核桃分心木多酚(Diaphragma juglandis Fructus polyphenols,DJF-P)的开发,该文通过对比5种大孔树脂对DJF-P静态吸附解吸效果,筛选出AB-8型大孔树脂最适于纯化DJF-P溶液,同时优化大孔树脂纯化的单因素试验条件,并通过高效液相色谱法(high performance liquid chromatography,HPLC)进一步对DJF-P溶液纯化前后的物质组成成分进行分析。结果表明:最佳提取条件为55%乙醇,料液比为1∶15(g/mL),室温下浸提24 h,浓缩,得到DJF-P浓缩液。最佳纯化工艺为55%乙醇稀释DJF-P浓缩液20倍,AB-8型大孔树脂静态吸附解吸时间均为8 h,解吸液为70%乙醇。HPLC结果表明,DJF-P含有槲皮素、阿魏酸、芦丁、绿原酸和没食子酸。
In order to promote the development of Diaphragma juglandis Fructus polyphenols(DJF-P),This study adopted a comparative analysis of five types of macroporous resins regarding their static adsorption-desorption performance for DJF-P.AB-8 macroporous resin was selected as the most suitable resin for purification of DJF-P solutions,and the single-factor experimental conditions for macroporous resin purification were optimized.The compositional components of DJF-P solutions before and after purification was further analyzed by high performance liquid chromatography(HPLC).The results indicated that the optimal extraction conditions were 55%ethanol at a solid-liquid ratio of 1∶15(g/mL),extraction at room temperature for 24 h,and subsequent concentration to obtain DJF-P concentrate.The optimal purification process included diluting the concentrated DJF-P solution with 55%ethanol at a 20 fold dilution,with an 8 h of static adsorption-desorption time using AB-8 macroporous resin,and a desorption solution comprising 70%ethanol.HPLC results showed that DJF-P contained quercetin,ferulic acid,rutin,chlorogenic acid,and gallic acid.
作者
许欢欢
苗春霖
宋颖
何爱民
吉洋洋
牟德华
高山
XU Huanhuan;MIAO Chunlin;SONG Ying;HE Aimin;JI Yangyang;MOU Dehua;GAO Shan(College of Food and Biology,Hebei University of Science and Technology,Shijiazhuang 050018,Hebei,China;Hebei Lüling Kangwei Food Co.,Ltd.,Xingtai 054300,Hebei,China;Hebei Province(Xingtai)Walnat Industry Technology Research Institute,Xingtai 054300,Hebei,China)
出处
《食品研究与开发》
CAS
北大核心
2023年第24期144-150,共7页
Food Research and Development
关键词
分心木
大孔树脂
多酚
纯化
组成
Diaphragma juglandis Fructus
macroporous resin
polyphenol
purification
composition
