摘要
近年来根肿病爆发式蔓延,对十字花科作物生产造成了严重威胁,以油菜为例,据不完全统计至2022年底全国发病已超133万公顷。培育抗病品种是阻止根肿病快速蔓延最经济有效的方式,而目前使用的抗性位点只有PbBa8.1和CRb,面对不同地区复杂多样的根肿菌和随时产生的新的变异类型,需要不断更新抗性种质资源。本研究通过接菌鉴定筛选了14份抗根肿病大白菜,根据已有的抗性位点相关标记鉴定发现DG117、ZS105、SG01、SR06、JQ07五份大白菜中不含CRb位点,且抗性强于华双5R/华油杂5R;含有CRb位点的KL02对PbXM(新民菌)的抗性表现强于现有材料409R,可能含有新的优异抗性位点。以上材料可作为新的根肿病抗性资源应用于甘蓝型油菜的抗根肿病新品种选育,为现有抗性资源做出补充。
Clubroot disease has spread explosively in recent years,posing a serious threat to cruciferous crop production.Take rapeseed for example,according to the statistics,until year 2022 the incidence of the clubroot has exceeded 1.33 million hectares in China.Breeding for resistant cultivars is considered as the most efficient and eco-nomical way to prevent clubroot disease spreading.However,the current CR cultivars mostly used CR loci of PbBa8.1 or CRb.Due to the complexity and diversity of Plasmodiophora brassicae from different locations,resources for resistant germplasm need to be explored.In this study,14 clubroot-resistant Chinese cabbages were character-ized using different clubroot pathogens and molecular markers for known CR loci.We found that among them,five materials,DG117,ZS105,SG01,SR06 and JQ07 did not contain CRb locus,and their disease resistance were stron-ger than Huashuang5R/Huayouza5R.At last,KL02 containing the CRb locus showed stronger resistance to PbXM(strain from Xinmin)than 409R,therefore it may contain other novel loci leading to better resistance.These six Chi-nese cabbage resources can be considerd as new resistance resources and could be utilized in future breeding work for CR Brassica napus.
作者
庾文琳
贺小芹
陈伟
罗佳杰
刘亚俊
刘应楠
史亚茹
杨露
向媛媛
杨庭芝
李根泽
陈鹏
张椿雨
YU Wen-lin;HE Xiao-qin;CHEN Wei;LUO Jia-jie;LIU Ya-jun;LIU Ying-nan;SHI Ya-ru;YANG Lu;XIANG Yuan-yuan;YANG Ting-zhi;LI Gen-ze;CHEN Peng;ZHANG Chun-yu(College of Plant Science and Technology,Huazhong Agricultural University,Wuhan 430070,China;JingmenAgricultural Technology Extension Center,Jingmen 448000,China;Agricultural Technology Extension Station ofLinxiang,Lincang 677000,China;Agricultural Technology Extension Station of Lincang,Lincang 677000,China;Plant Protection and Inspection Station of Linxiang,Lincang 677000,China;Industrial Crops Instituteof Yunnan Agricultural Academy of Science,Kumming 650205,China)
出处
《中国油料作物学报》
CAS
CSCD
北大核心
2023年第6期1141-1149,共9页
Chinese Journal of Oil Crop Sciences
基金
湖北省科技重大专项(2022ABA001)
2022年可持续发展议程创新示范区科技专项(202204AC100001-A04)
国家现代农业产业技术体系建设专项(CARS-12)。
关键词
油菜
根肿病
抗根肿病大白菜
种质筛选
分子标记
Brassica napus
clubroot
CR Chinese cabbages
screening germplasm resources
molecular marker
