摘要
【目的】明确紫外线辐照被寄生的寄主蛹对寄生蜂果蝇锤角细蜂Trichopria drosophilae生长发育的影响,以期为改进大量繁殖果蝇锤角细蜂的技术提供参考。【方法】在暗箱中,分别使用UVA,UVB和UVC 3种紫外灯辐照处理被寄生的黑腹果蝇Drosophila melanogaster蛹,辐照时长分别为3,6和9 h,观测辐照后果蝇锤角细蜂的死蛹率、卵-成蜂历期、成蜂羽化率和性比(雌雄性比)及寿命等指标。【结果】与对照相比,UVA辐照对果蝇锤角细蜂的成蜂羽化率、死蛹率和雌雄性比均无显著影响;UVB辐照3,6和9 h以及UVC辐照3和9 h时,果蝇锤角细蜂的成蜂羽化率显著降低,死蛹率显著升高;UVB辐照3,6和9 h时,寄生蜂雌雄性比均大于2.00。UVA辐照3和6 h以及UVC辐照3,6和9 h时,果蝇锤角细蜂卵-雌成蜂历期显著低于对照。UVC辐照3,6和9 h时,果蝇锤角细蜂卵-雄成蜂历期显著低于对照。随UVA辐照时间的增加雌成蜂寿命增加,但UVA辐照组雌成蜂寿命与对照组均无显著差异,UVB辐照6 h时雌成蜂寿命显著高于对照;经过紫外线辐照处理后雄成蜂寿命均高于对照,UVA辐照3 h、UVB辐照6 h和UVC辐照9 h时雄成蜂寿命与对照差异显著。【结论】紫外线辐照被寄生的黑腹果蝇蛹可以显著影响果蝇锤角细蜂的卵-成蜂历期和成蜂寿命。本研究结果为改进果蝇害虫生物防治天敌资源的大量扩繁技术提供参考。
【Aim】To clarify the effects of ultraviolet radiation to the parasitized host pupa on the growth and development of the parasitoid Trichopria drosophilae,so as to provide a reference for the mass breeding of T.drosophilae.【Methods】Three ultraviolet irradiation lamps,UVA,UVB and UVC,were used to irradiate the parasitized Drosophila melanogaster pupae in a dark box.The irradiation duration was 3,6 and 9 h,respectively.The pupal mortality rate,egg-adult duration,adult emergence rate,sex ratio(female to male ratio),and longevity of T.drosophilae were observed and calculated.【Results】Compared to the control,UVA irradiation showed no significant effects on the adult emergence rate,pupal mortality rate and female to male ratio of T.drosophilae.After UVB irradiation for 3,6 and 9 h,and UVC irradiation for 3 and 9 h,the adult emergence rates of T.drosophilae decreased significantly and the pupal mortality rates increased significantly compared to the control.The female to male ratios of T.drosophilae irradiated by UVB for 3,6 and 9 h were greater than 2.00.After UVA irradiation for 3 and 6 h,and UVC irradiation for 3,6 and 9 h,the egg-female adult duration of T.drosophilae was significantly lower than that of the control.After UVC irradiation for 3,6 and 9 h,the egg-male adult duration of T.drosophilae was significantly lower than that of the control.The female adult longevity of T.drosophilae raised with the increase of irradiation duration of UVA,but showed no significant difference between the UVA irradiation groups and the control.The female adult longevity in UVB irradiation for 6 h was significantly higher than that in the control.The male adult longevity of T.drosophilae in ultraviolet irradiation treatments was higher than that of the control,and the male adult longevity in UVA irradiation for 3 h,UVB irradiation for 6 h and UVC irradiation for 9 h exhibited significant difference from that in the control.【Conclusion】Ultraviolet irradiation treatment on the parasitized D.melanogaster pupae can significantly affect the egg-adult duration and adult longevity of T.drosophilae.The results of this study provide a reference for the multiplication of natural enemy resources for biological control of Drosophila pests.
作者
刘旭祥
杨永棒
凡庆文
张沁园
季清娥
LIU Xu-Xiang;YANG Yong-Bang;FAN Qing-Wen;ZHANG Qin-Yuan;JI Qing-E(UN(China)Center for Fruit Fly Prevention and Treatment,Key Laboratory of Biopesticide and Chemical Biology,Ministry of Education,State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops,Biological Control Research Institute,Fujian Agriculture and Forestry University,Fuzhou 350002,China)
出处
《昆虫学报》
CAS
CSCD
北大核心
2023年第11期1518-1526,共9页
Acta Entomologica Sinica
基金
国家重点研发计划项目(2023YFD1400700)
IAEA CRP项目(D41030)。
关键词
果蝇锤角细蜂
黑腹果蝇
紫外线辐照
生长发育
生物防治
Trichopria drosophilae
Drosophila melanogaster
ultraviolet irradiation
growth and development
biological control
