摘要
目的 基于新的检测机制,构建胺类气体的高灵敏度和高选择性传感器。方法 以2,5-二氨基-1,4-苯二噻吩二盐酸盐、羟基对苯二甲酸和1,10-十二羧酸为原料通过脱水反应制备得到具有激发态分子内质子转移(excited-state intramolecular proton transfer, ESIPT)特性的聚合物,并采用红外光谱对其进行结构表征。通过ESIPT聚合物与胺蒸气的选择性切断乙酰基释放出产生荧光的基团,建立胺蒸气浓度与荧光强度之间的关系,实现食品腐败的高灵敏检测。结果 制备得到ESIPT聚合物具有大的斯托克斯位移(160nm)。在400 nm光照射下, ESIPT聚合物在542 nm处的荧光强度与胺蒸气浓度之间具有良好的线性关系,相关系数为0.98,检出限为11mg/L并具有高选择性。相比较于低温保存下的牛肉,储存于25℃下牛肉的ESIPT薄膜荧光强度更强。结论 该传感器利用其轻便性和对胺蒸气的高灵敏度,可通过检测微生物生长产生的挥发性胺来用于食品腐败监测。
Objective To construct high sensitivity and high selectivity sensors of amine gas based on new detection mechanism.Methods Polymers with excited-state intramolecular proton transfer(ESIPT)characteristics were prepared by dehydration reaction among 2,5-diamino-1,4-benzothiophene dihydrochloride,hydroxyterephthalate and 1,10-dodecarboxylic acid,whose structure was determined by infrared spectroscopy.The fluorescence-producing groups were released by selective severing of the acetyl group in the ESIPT polymer with the amine vapor,relationship between the amine vapor concentration and the fluorescence intensity was thereby established and a highly sensitive detection of food spoilage was achieved.Results ESIPT polymer exhibited a large Stokes shift(160 nm).Under excitation of 400 nm,the fluorescence intensity of as-prepared ESIPT polymers at 542 nm and amine vapor concentration showed a good linear relationship with a correlation coefficient of 0.98,simultaneously,a limit of detection of 11 mg/L and high selectivity was achieved.The fluorescent intensity of ESIPT films exposed to beef stored at 25℃were stronger compared with cry preserved one.Conclusion Due to its portability and high sensitivity to amine vapor,this sensor can be used in food spoilage by detecting volatile amines produced by microbial growth.
作者
鲁静
谢一民
王璇
LU Jing;XIE Yi-Min;WANG Xuan(College of Chemistry and Bioengineering,Yinchuan Energy Institute,Yinchuan 750105,China;Ningxia Testing Laboratory of Coal Chemical,Yinchuan 750002,China)
出处
《食品安全质量检测学报》
CAS
北大核心
2023年第22期220-225,共6页
Journal of Food Safety and Quality
基金
宁夏自然科学基金项目(2020AAC03275)。
关键词
激发态分子内质子转移聚合物
荧光传感器
胺蒸气
食品安全
excited-state intramolecular proton transfer polymer
fluorescent sensor
amine vapor
food safety
