摘要
为了挖掘影响山西道地药材潞党参道地性形成的转录因子,该研究基于山西潞党参与甘肃白条党转录组数据筛选潞党参特异性转录因子,并对其进行基因表达模式分析,为通过分子辅助育种进行潞党参优种选育提供理论基础。根据不同产地潞党参与白条党转录组数据,对差异转录因子进行GO功能注释、鉴定、保守基序分析及基因表达模式分析。自转录组数据库中共筛选出61个差异基因,分属最多的AP2/ERF-ERF家族的保守基序为[2X]-[LG]-[3X]-T-[3X]-[AARAYDRAA]-[3X]-[RG]-[2X]-A-[2X]-[NFP]。分析发现,差异基因中43个在潞党参中表达量显著高于白条党,包括AP2/ERF-ERF家族11个、NAC家族5个、bHLH家族1个、RWP-RK家族2个,而有18个转录因子基因在白条党中上调表达。转录结合活性、代谢进程、应激反应相关的GO terms分别为103、26、23个。不同器官特异性表达分析表明转录因子基因CpNAC92、CpNAC100、CpbHLH128、CpRAP2-7不仅在潞党参中上调表达,在根系中表达量也显著高于其他器官。环境胁迫条件下基因表达模式分析发现,除CpNAC100基因只响应低温、过铁胁迫外,其他3个基因均可响应低温、温差、过铁胁迫。潞党参特异性转录因子基因CpNAC92、CpNAC100、CpbHLH128、CpRAP2-7的筛选与表达分析,为进一步研究潞党参道地性形成机制奠定了理论基础。
This study aims to mine the transcription factors that affect the genuineness of Codonopsis pilosula in Shanxi based on the transcriptome data of C.pilosula samples collected from Shanxi and Gansu,and then analyze the gene expression patterns,which will provide a theoretical basis for the molecular assisted breeding of C.pilosula.Gene ontology(GO) functional annotation,conserved motif prediction,and gene expression pattern analysis were performed for the differential transcription factors predicted based on the transcriptome data of C.pilosula from different habitats.A total of 61 differentially expressed genes(DEGs) were screened out from the transcriptome data.Most of the DEGs belonged to AP2/ERF-ERF family,with the conserved motif of [2X]-[LG]-[3X]-T-[3X]-[AARAYDRAA]-[3X]-[RG]-[2X]-A-[2X]-[NFP].Forty-three of the DEGs showed significantly higher gene expression in C.pilosula samples from Shanxi than in the samples from Gansu,including 11 genes in the AP2/ERF-ERF family,5 genes in the NAC fa-mily,1 gene in the bHLH family,and 2 genes in the RWP-RK family,while 18 transcription factors showed higher expression levels in the samples from Gansu.GO annotation predicted that most of the DEGs were enriched in GO terms related to transcriptional binding activity(103),metabolic process(26),and stress response(23).The expression of transcription factor genes,CpNAC92,CpNAC100,CpbHLH128,and CpRAP2-7 was higher in the samples from Shanxi and in the roots of C.pilosula.CpNAC92,CpbHLH128,and CpRAP2-7 responded to the low temperature,temperature difference,and iron stresses,while CpNAC100 only responded to low temperature and iron stresses.The screening and expression analysis of the specific transcription factors CpNAC92,CpNAC100,CpbHLH128,and CpRAP2-7 in C.pilosula in Shanxi laid a theoretical foundation for further research on the mechanism of genuineness formation of C.pilosula.
作者
翟宇佳
戴俊利
刘幸
田星锐
吉姣姣
高建平
ZHAI Yu-jia;DAI Jun-li;LIU Xing;TIAN Xing-rui;JI Jiao-jiao;GAO Jian-ping(School of Pharmaceutical Science,Shanxi Medical University,Taiyuan 030001,China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2023年第21期5779-5789,共11页
China Journal of Chinese Materia Medica
基金
国家自然科学基金项目(82204569)
山西省自然科学基金项目(202103021224225)
中央本级重大增减支项目(2060302)
国家重点研发计划项目(2019YFC1710800)。
关键词
潞党参
转录组
差异转录因子
表达分析
Codonopsis pilosula in Shanxi
transcriptome
differential transcription factor
expression analysis
