摘要
植物源外泌体样纳米颗粒(plant-derived exosome-like nanoparticles,PELNs)是从植物组织中分离得到的一类直径为30~300 nm的膜性小泡,其内含有蛋白质、脂质和核酸等成分,在植物自身物质代谢、免疫防御等方面具有重要作用,并且还能够跨界调控真菌、动物细胞的生理活动,具有极大的应用价值。近年来,PELNs的研究急剧增多,越发凸显了3个方面问题:(1)PELNs的植物材料来源混杂;(2)缺乏统一的PELNs分离与表征技术体系;(3)PELNs的生物跨界调控分子机制亟待阐明。该文聚焦于上述问题,首先概述了PELNs的生物发生和组成成分,并讨论了PELNs的分离技术、表征方法,同时对PELNs在生物医学上的应用以及未来的研究方向进行了分析,以期推动PELNs研究规范的建立,为深入解析PELNs的跨界调控作用机制提供理论参考。
Plant-derived exosome-like nanoparticles(PELNs) are a class of membranous vesicles with diameters approximately ranging from 30 to 300 nm,isolated from plant tissues.They contain components such as proteins,lipids,and nucleic acids.PELNs play an important role in the metabolism of plant substances and immune defense,and can also cross-regulate the physiological activities of fungi and animal cells,showing significant potential applications.In recent years,research on PELNs has significantly increased,highlighting three main issues:(1) the mixed sources of plant materials for PELNs;(2) the lack of a unified system for isolating and characterizing PELNs;(3) the urgent need to elucidate the molecular mechanisms underlying the cross-regulation of biological functions by PELNs.This article focused on these concerns.It began by summarizing the biological origin and composition of PELNs,discussing the techniques for isolating and characterizing PELNs,and analyzing their biomedical applications and potential future research directions.,aiming to promote the establishment of standardized research protocols for PELNs and provide theoretical references for in-depth exploration of the mechanisms underlying PELNs′ cross-regulatory effects.
作者
潘林思
王文彩
姚孟宇
王晓燕
张宪智
PAN Lin-si;WANG Wen-cai;YAO Meng-yu;WANG Xiao-yan;ZHANG Xian-zhi(College of Horticulture and Landscape Architecture,Zhongkai University of Agriculture and Engineering,Guangzhou 510225,China;Science and Technology Innovation Center,Guangzhou University of Chinese Medicine,Guangzhou 510405,China;Department of Orthopedics,Guangdong Provincial People′s Hospital,Guangdong Academy of Medical Science,Southern Medical University,Guangzhou 510000,China;Doctor Workstation,Lianjiang People′s Hospital Affiliated to Guangdong Medical University,Zhanjiang 524400,China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2023年第22期5977-5984,共8页
China Journal of Chinese Materia Medica
基金
广东省重点领域研发计划项目(2020B020215003,2021B0707010001)
广东省林业科技创新项目(2021KJCX015)
广东省普通高校优稀特色经济林果工程技术研究中心项目(2022GCZX002)。
关键词
外泌体
植物源外泌体样纳米颗粒
分离与表征
跨界调控
医学应用
exosome
plant-derived exosome-like nanoparticles
separation and characterization
cross-regulation
medical application
