摘要
镉(Cd)是一种有毒的、流动性很强的重金属,可以被植物大量地吸附和吸收而没有任何明显的迹象。因此,对保护生物多样性和食品安全来说,在植物吸收之前稳定Cd是至关重要的。由于在其结构中含有大量的羧基和酚羟基,腐殖物质与重金属形成强烈的结合,这使它们成为完美的稳定剂。本研究的目的是确定在温室条件下,腐植酸和黄腐酸(HA+FA)的水平(0、3500、5250和7000 mg/L)对减轻独行菜(Lepidium sativum)受Cd(CdSO_(4)•8H_(2)O,0、100和200 Cd mg/kg)污染的Cd毒性的影响。我们的研究结果表明,Cd胁迫对独行菜的生长有负面影响,叶鲜重、叶干重、根鲜重和根干重、叶片相对含水量(LRWC)和除Cd外的矿物质含量均下降,膜渗透性(MP)和酶(CAT、SOD和POD)活性增加。然而,HA+FA的应用减少了Cd污染的不利影响。在200 mg/kg的Cd污染下,HA+FA的应用浓度为7000 mg/L时,与对照组相比,叶鲜重、叶干重、根鲜重、根干重、茎粗、叶面积、叶绿素读取值(CRV)、MP和LRWC值分别增加了262%、137%、550%、133%、92%、104%、34%、537%和32%。尽管在200 mg/L的镉污染下获得了最高的H2O2、丙二醛(MDA)、脯氨酸和蔗糖值,但HA+FA的应用浓度在200 mg/kg的Cd污染水平下,7000 mg/L的HA+FA成功地缓解了Cd胁迫的有害影响,使H2O2、MDA、脯氨酸和蔗糖值分别下降了66%、68%、70%和56%。浓度为7000 mg/L的HA+FA应用成功地缓解了Cd污染的负面影响,使N、P、K、Ca、Mg、Fe、Mn、Cu、Zn和B分别提高75%、23%、84%、87%、40%、85%、143%、1%、65%和115%。此外,HA+FA的应用浓度为7000 mg/L成功地减少了95%的Cd吸收和80%的Cl吸收。考虑到植物生长参数,当HA+FA浓度为7000 mg/L时,确定了最佳结果。我们已经证明,使用高比例的FA的腐殖质是非常关键的,FA的比例为10%,可以达到减轻重金属胁迫对植物生长不利影响的目的。总之,HA+FA的应用可以作为一种有效的解决方案,通过稳定土壤中的Cd,防止Cd从植物的根部转移到其嫩枝和叶片,从而减少植物对Cd的吸收。
Cadmium(Cd)is a toxic and very mobile heavy metal that can be adsorbed and uptaken by plants in large quantities without any visible sign.Therefore,stabilization of Cd before uptake is crucial to the conservation of biodiversity and food safety.Owing to the high number of carboxyl and phenolic hydroxyl groups in their structure,humic substances form strong bonds with heavy metals which makes them perfect stabilizing agents.The aim of this study was to determine the effects of humic and fulvic acid(HA+FA)levels(0,3500,5250,and 7000 mg/L)on alleviation of Cadmium(Cd)toxicity in garden cress(Lepidium sativum)contaminated with Cd(CdSO_(4)·8H_(2)O)(0,100,and 200 Cd mg/kg)under greenhouse conditions.Our results showed that,Cd stress had a negative effect on the growth of garden cress,decreased leaf fresh,leaf dry,root fresh and root dry weights,leaf relative water content(LRWC),and mineral content except for Cd,and increased the membrane permeability(MP)and enzyme(CAT,SOD and POD)activity.However,the HA+FA applications decreased the adverse effects of the Cd pollution.At 200 mg/kg Cd pollution,HA+FA application at a concentration of 7000 mg/L increased the leaf fresh,leaf dry,root fresh,root dry weights,stem diameter,leaf area,chlorophyll reading value(CRV),MP,and LRWC values by 262%,137%,550%,133%,92%,104%,34%,537%,and 32%respectively,compared to the control.Although the highest H2O2,MDA,proline and sucrose values were obtained at 200 mg/L Cd pollution,HA+FA application at a concentration of 7000 mg/L successfully alleviated the deleterious effects of Cd stress by decreasing H2O2,MDA,proline,and sucrose values by 66%,68%,70%,and 56%,respectively at 200 mg/kg Cd pollution level.HA+FA application at a concentration of 7000 mg/L successfully mitigated the negative impacts of Cd pollution by enhanced N,P,K,Ca,Mg,Fe,Mn,Cu,Zn,and B by 75%,23%,84%,87%,40%,85%,143%,1%,65%,and 115%,respectively.In addition,HA+FA application at a concentration of 7000 mg/L successfully reduced Cd uptake by 95%and Cl uptake by 80%.Considering the plant growth parameters,the best results were determined when HA+FA concentration was 7000 mg/L.We have shown that,it is critical to apply a humic substance with high percentage of FA,which was 10%in this study,to mitigate the adverse effects of heavy metal stress on plant growth.In conclusion,the application of HA+FA may be suggested as an effective solution for reducing the Cd uptake of the plants by stabilizing Cd in soil and preventing translocation of Cd from the roots of plant to its shoot and leaves.
作者
Ertan Yildirim
Melek Ekinci
Metin Turan
Güleray Ağar
Atilla Dursun
Raziye Kul
Zeynep Alim
Sanem Argin
牛华琳(译)
庄琪(译)
Ertan Yildirim;Melek Ekinci;Metin Turan;Güleray Ağar;Atilla Dursun;Raziye Kul;Zeynep Alim;Sanem Argin;Niu Hualin;Zhuang Qi(Department of Horticulture,Faculty of Agriculture,Atatürk University,Erzurum,Turkey,25240;Department of Genetics and Bioengineering,Yeditepe University,Istanbul,Turkey,34755;Department of Biology,Faculty of Science,Atatürk University,Erzurum,Turkey,25240;Department of Horticulture and Agronomy,Kyrgyz-Turkish Manas University,Bishkek,Kyrgyz Republic,720001;Department of Agricultural Trade and Management,Yeditepe University,Istanbul,Turkey,34755;College of Horticulture,Shanxi Agricultural University,Jinzhong,030600)
出处
《腐植酸》
CAS
2023年第6期68-76,共9页
Humic Acid
关键词
镉
腐植酸
黄腐酸
植物生长
生理生化特性
独行菜
cadmium
humic acid
fulvic acid
plant growth
physiology and biochemical properties
garden cress
