摘要
旨在了解牦牛铁蛋白轻链(FTL)基因的结构与功能,以及在不同年龄段8个组织中的表达规律。以成年(36月龄)美仁牦牛肝脏组织cDNA为模板,克隆FTL基因的编码区序列,并进行生物信息学分析,利用实时荧光定量(qRT-PCR)技术检测FTL基因在心脏、肝脏、脾脏、肺脏、肾脏、背最长肌、脂肪、睾丸中的相对表达量。结果表明,美仁牦牛FTL基因的CDS区为528 bp,共编码175个氨基酸,与野牦牛和牛的亲缘关系最近(99.8%);FTL蛋白为稳定亲水性分泌蛋白,不存在信号肽和跨膜结构,主要分布在细胞质中,蛋白的二级结构以α-螺旋为主,主要与FTH1、SCARA5、NCOA4等蛋白存在相互作用。qRT-PCR结果显示,FTL基因在成年牛的表达量依次为肝脏、肾脏、睾丸,脾脏、肺脏、脂肪、背最长肌、心脏,在7日龄犊牛的脂肪和背最长肌组织中表达最高,成年牛中肝脏组织表达量最高;通过2个阶段比对发现,脂肪、背最长肌和心脏组织的表达量随美仁牦牛年龄的增长呈下降趋势,而肺脏、脾脏、睾丸、肾脏和肝脏组织的表达量随年龄的增长而升高。
The aim of this study was to understand the structure and function of ferritin light chain(FTL)gene in yak and its expression pattern in eight tissues of different age groups.The coding sequence(CDS)of FTL gene was cloned from the cDNA of liver tissue of adult(36 months old)Meiren yak,and bioinformatics analysis was carried out.The relative expression of FTL gene in heart,liver,spleen,lung,kidney,longissimus dorsi,fat and testis was detected by Real-time fluorescence quantitative(qRT-PCR).The results showed that the CDS region of FTL gene of Meiren yak was 528 bp,encoding 175 amino acids,and had the closest relationship with wild yak and cattle(99.8%).FTL protein was a stable hydrophilic secretory protein without signal peptide and transmembrane structure.It was mainly distributed in the cytoplasm.The secondary structure of the protein was mainlyα-helix,which mainly interacted with FTH1,SCARA5,NCOA4 and other proteins.The results of qRT-PCR showed that the expression levels of FTL gene in adult cattle were liver,kidney,testis,spleen,lung,fat,longissimus dorsi and heart,respectively.The expression levels of FTL gene in fat and longissimus dorsi tissues of 7-day-old calves were the highest,and the expression level of FTL gene in liver tissue of adult cattle was the highest.By comparing the two stages,it was found that the expression levels of fat,longissimus dorsi and heart tissues decreased with the increase of age,while the expression levels of lung,spleen,testis,kidney and liver tissues increased with age.
作者
张娟香
马小勇
路建卫
仁青吉
罗胜伟
肖光峰
王有鹤
马晓明
喇永福
郭宪
褚敏
阎萍
梁春年
ZHANG Juanxiang;MA Xiaoyong;LU Jianwei;REN Qingji;LUO Shengwei;XIAO Guangfeng;WANG Youhe;MA Xiaoming;LA Yongfu;GUO Xian;CHU Min;YAN Ping;LIANG Chunnian(Lanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agricultural Sciences,Key Laboratory of Yak Breeding Engineering of Gansu Province,Lanzhou 730050,China;College of Animal Science and Technology,Gansu Agricultural University,Lanzhou 730070,China;Key Laboratory of Animal Genetics and Breeding on the Tibetan Plateau,Ministry of Agriculture and Rural Affairs,Lanzhou 730050,China;Animal Husbandry Station of Zogaidoma Township,Hezuo,Hezuo 747000,China;Hezuo City Animal Disease Prevention and Control Center,Hezuo 747099,China)
出处
《华北农学报》
CSCD
北大核心
2023年第6期192-200,共9页
Acta Agriculturae Boreali-Sinica
基金
甘肃省科技计划项目(20JR5RA580)
甘肃省科技重大项目(21ZD10NA001,GZGG-2021-1)
合作市牦牛种质提升与提质增效项目、现代农业(肉牛牦牛)产业技术体系建设专项资金(CARS-37)
中国农业科学院科技创新工程(25-LZIHPS-01)。
