摘要
目的观察麦粒灸“环跳”对坐骨神经损伤(SNI)大鼠坐骨神经功能、坐骨神经干病理形态及脊髓组织TLR4/MyD88/NF-κB表达的影响,探究麦粒灸治疗SNI的可能机制。方法24只雄性SD大鼠随机分为空白组、假手术组、模型组和麦粒灸组,每组6只。模型组、麦粒灸组采用坐骨神经钳夹制备SNI大鼠模型,造模后第7日起麦粒灸组取患侧“环跳”麦粒灸干预,每次6壮,1次/d,连续10 d。观察造模后第7日及干预结束后大鼠坐骨神经功能指数(SFI)、纤维丝测痛仪测量大鼠机械痛阈值(MWT),ELISA检测脊髓组织一氧化氮(NO)、诱导型一氧化氮合酶(iNOS)含量,HE染色观察坐骨神经干形态,Western blot检测脊髓组织Toll样受体4(TLR4)、核因子(NF)-κBp65、p-NF-κBp65、髓样分化因子88(MyD88)、NF-κB抑制蛋白α(IκBα)、p-IκBα蛋白表达。结果与假手术组比较,模型组大鼠SFI、MWT显著降低(P<0.01),坐骨神经干神经纤维排列紊乱,施万细胞数量明显增多,有大量空泡变性,脊髓组织NO、iNOS含量及TLR4、p-NF-κBp65、MyD88、p-IκBα蛋白表达显著升高(P<0.01);与模型组比较,麦粒灸组大鼠SFI、MWT显著升高(P<0.01),坐骨神经干损伤减轻,细胞排列较整齐,施万细胞数量减少,轴突脱髓鞘及细胞空泡变性减少,脊髓组织NO、iNOS含量及TLR4、p-NF-κBp65、MyD88、p-IκBα蛋白表达显著降低(P<0.05)。结论麦粒灸“环跳”可下调SNI大鼠脊髓组织TLR4、p-NF-κBp65、MyD88、p-IκBα蛋白表达,抑制NO、iNOS分泌,从而缓解疼痛、减轻受损神经组织炎症反应。
Objective To observe the effects of wheat grain moxibustion for“Huantiao”on sciatic nerve function,pathological morphology of sciatic nerve stem and expressions of TLR4/MyD88/NF-κB signaling pathway expression in spinal cord tissue of rats with sciatic nerve injury(SNI);To explore the possible mechanism of wheat grain moxibustion for the treatment of SNI.Methods Totally 24 SD male rats were randomly divided into blank group,sham-operation group,model group and wheat grain moxibustion group,with 6 rats in each group.The model group and the wheat grain moxibustion group used a rat model with sciatic nerve clamping injury.From the 7th day after modeling,the rats were treated with moxibustion on the affected side of“Huantiao”for 6 strokes each time,once a day,for consecutive 10 days.The sciatic nerve function index(SFI)of rats on the 7th day after modeling and after intervention were observed,mechanical withdraw threshold(MWT)in rats were measured using a fiber optic pain gauge,ELISA was used to detect NO and iNOS content in spinal cord tissue,HE staining was used to observe the morphology of sciatic nerve stem,the expression of TLR4,NF-κBp65,p-NF-κBp65,MyD88,IκBαand p-IκBαin spinal cord tissue were detected by Western blot.Results Compared with the sham-operation group,the SFI and MWT of the rats in the model group significantly decreased(P<0.01),the arrangement of nerve fibers in sciatic nerve stem was disordered,with a significant increase in the number of Schwann cells and a large number of vacuolar degeneration,the content of NO,iNOS and the expression of TLR4,p-NF-κBp65,MyD88,p-IκBαprotein in spinal cord tissue significantly increased(P<0.01).Compared with the model group,the SFI and MWT of the rats in the wheat grain moxibustion group increased significantly(P<0.01),the damage of sciatic nerve stem was reduced,with orderly cell arrangement,a decrease in the number of Schwann cells,and a decrease in axonal demyelination and cellular vacuolar degeneration,the content of NO,iNOS and the expression of TLR4,p-NF-κBp65,MyD88,p-IκBαin spinal cord tissue significantly decreased(P<0.05).Conclusion Wheat grain moxibustion for“Huantiao”can down-regulate TLR4,p-NF-κBp65,MyD88 and p-IκBαprotein expressions in spinal cord tissue of SNI rats,reduce the secretion of NO and iNOS,thereby relieve pain and damaged nerve tissue inflammation response.
作者
苏虹
张熙
李竞
郭彦君
粟胜勇
羊璞
王琼潇
谢彩云
SU Hong;ZHANG Xi;LI Jing;GUO Yanjun;SU Shengyong;YANG Pu;WANG Qiongxiao;XIE Caiyun(First Clinical Medical College of Guangxi University of Chinese Medicine,Nanning 530001,China;The First Affiliated Hospital of Guangxi University of Chinese Medicine,Nanning 530023,China)
出处
《中国中医药信息杂志》
CAS
CSCD
2024年第1期78-83,共6页
Chinese Journal of Information on Traditional Chinese Medicine
基金
国家自然科学基金(82160934、81960895)
广西中医药大学桂派杏林拔尖人才项目(2022C017)
广西中医药重点学科建设项目(GZXK-Z-20-38)。
