摘要
目的 基于网络药理学方法探讨散结通脉方化裁方治疗动脉粥样硬化潜在作用机制。方法 从TCMSP数据库检索散结通脉方化裁方活性成分及其靶点;通过GeneCards、OMIM和DrugBank数据库收集动脉粥样硬化相关靶点;将药物与疾病靶点取交集;使用STRING数据库构建交集靶点蛋白相互作用(PPI)网络并通过Cytoscape3.7.2软件筛选关键靶点。利用在线软件DAVID进行GO功能和KEGG富集分析。结果 检索并筛选获得散结通脉方化裁方活性成分85个,潜在靶点648个,动脉粥样硬化疾病相关靶点1 439个,获得交集靶点131个;筛选得到散结通脉方化裁方治疗动脉粥样硬化疾病的关键成分5个,包括槲皮素、木犀草素、人参皂苷Rg2、丹参酮ⅡA、隐丹参酮等;PPI网络显示关键靶点为VEGFA、AKT1、STAT3、TNF、IL6等;GO富集共得出631条生物过程,104条分子功能和61条细胞组分;KEGG通路富集分析共得出153条,包括癌症的途径、脂质与动脉粥样硬化、PI3K-Akt信号通路、糖尿病并发症中的AGE-RAGE信号通路、流体切应力和动脉粥样硬化等。结论 本研究初步揭示了散结通脉方化裁方多成分、多靶点、多途径治疗动脉粥样硬化的科学内涵,为该方的临床开发利用提供了依据,并进一步深化“瘀能化水”理论认识。
Objective To explore the potential mechanism of Modified Sanjie Tongmai Decoction for the treatment of atherosclerosis based on modified network pharmacological.Methods The active components and targets of modified Sanjie Tongmai Decoction were screened from TCMSP database;atherosclerosis related targets were collected through GeneCards,OMIM and DrugBank databases;the drug and the disease targets were cross mapped to obtain the treatment target;the STRING database were used for protein-protein interaction(PPI)network construction and key targets were screened through the Cytoscape 3.7.2 software.The online software DAVID was used for GO and KEGG enrichment analysis.Results Totally 85 active components from modified Sanjie Tongmai Decoction, 648 potential targets, 1 439 atherosclerotic disease related target and 131 cross targets were obtained by retrieving and screening;5 key active components of modified Sanjie Tongmai Decoction for atherosclerotic diseases were screened, including quercetin, luteolin, ginsenoside Rg2, tanshinone ⅡA, cryptotanshinone, etc.;PPI showed that the potential key targets were VEGFA, AKT1, STAT3, TNF, IL6 etc.;631 biological processes, 104 molecular functions and 61 cell components were obtained by GO enrichment;KEGG pathway enrichment analysis obtained 153 results, including cancer pathway, lipid and atherosclerosis, PI3K-Akt signaling pathway, AGE-RAGE signaling pathway in diabetis complications, fluid shear stress and atherosclerosis. Conclusion This study preliminarily reveals the scientific connotation of modified Sanjie Tongmai Decoction for the treatment of atherosclerosis with multi components, multi targets and multi approaches, which can provide a basis for the clinical development and utilization of the decoction, and further deepen the theoretical understanding of “blood stasis can transform into water”.
作者
邱萌萌
李双娣
QIU Mengmeng;LI Shuangdi(Changchun University of Chinese Medicine,Changchun 130117,China;Affiliated Hospital of Changchun University of Chinese Medicine,Changchun 130021,China)
出处
《中国中医药图书情报杂志》
2024年第1期76-82,共7页
Chinese Journal of Library and Information Science for Traditional Chinese Medicine
基金
吉林省卫生与健康技术创新项目(2020J066)。
