摘要
目的:探讨急慢性肩袖损伤患者肩袖组织的促氧化状态及Beclin-1、哺乳动物雷帕霉素靶蛋白(mammalian target of rapamycin,mTOR)的表达水平,分析肩袖损伤与氧化应激和自噬的关系。方法:选取2019年7月至2020年12月行肩关节镜下肩袖修复术治疗的40例肩袖损伤患者,分为男性慢性损伤组、男性急性损伤组、女性慢性损伤组和女性急性损伤组,每组10例。术中提取肩袖损伤部位的肌腱样本,检测其总活性氧(reactive oxygen species,ROS)及超氧化物歧化酶(superoxide dismutase,SOD)水平;反转录酶-聚合酶链锁反应(reverse transcription-polymerase chain reaction,RT-PCR)技术检测Beclin-1、mTOR mRNA的表达情况,Western-blot法检测Beclin-1、p-mTOR/mTOR蛋白的表达。结果:男性与女性慢性损伤组、男性与女性急性损伤组ROS、SOD以及Beclin-1、mTOR mRNA的表达比较,差异无统计学意义(P>0.05);男性慢性损伤组较急性损伤组ROS、SOD以及Beclin-1基因和蛋白表达水平明显上调,而mTOR基因和蛋白表达水平明显下调(P<0.05);女性慢性损伤组较急性损伤组ROS、SOD以及Beclin-1基因和蛋白表达水平明显上调,而mTOR基因和蛋白表达水平明显下调(P<0.05)。结论:慢性肩袖损伤比急性肩袖损伤更容易激发肩袖组织促氧化状态,上调自噬因子Beclin-1的表达、下调mTOR的表达。慢性肩袖损伤患者氧化应激反应和自噬水平可能更高。
Objective To explore pro-oxidative state of rotator cuff tissue and expression levels of Beclin-1 and mammalian target of rapamycin(mTOR) in patients with acute and chronic rotator cuff injury,and then analyzed relationship between rotator cuff injury and oxidative stress and autophagy.Methods Forty patients with rotator cuff injury were seleceted from July 2019 to December 2020,and divided into male chronic injury group,male acute injury group,female chronic injury group,and female acute injury group,10 patients in each group.All patients were performed rotator cuff repair under arthroscopy.The sample of tendon at the rotator cuff injury site of the patient was taken during operation,and total reactive oxygen species(ROS) and superoxide dismutase(SOD) were detected by detection kit;expression of Beclin-1 and mTOR mRNA were detected by reverse transcription-polymerase chain reaction(RT-PCR),and Western-blot was applied to detect protein expression of Beclin-1 and p-mTOR/mTOR.Results There were no significant difference in expression of ROS,SOD,Beclin-1mRNA and mTOR mRNA between male and female chronic injury groups,and between male and female acute injury groups(P>0.05);ROS,SOD and Beclin-1mRNA in male chronic injury group were higher than those in male chronic injury group,while mTOR mRNAand protein decreased(P<0.05);ROS,SOD and Beclin-1 mRNA in female chronic injury group were up-regulated compared with female acute injury group,while mTOR mRNA was down-regulated(P<0.05).Conclusion Chronic rotator cuff injury is more likely to stimulate the pro-oxidation state of rotator cuff tissue than acute rotator cuff injury,which could up-regulating expression of autophagy factor Beclin-1 and down-regulating expression of mTOR.Therefore,patients with chronic rotator cuff injury may have higher levels of oxidative stress and autophagy.
作者
刘治军
魏合伟
郑维蓬
万雷
廖志浩
陈胜
LIU Zhi-jun;WEI He-wei;ZHENG Wei-peng;WAN Lei;LIAO Zhi-hao;CHEN Sheng(The Third Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou 510240,Guangdong,China)
出处
《中国骨伤》
CAS
CSCD
2023年第12期1136-1141,共6页
China Journal of Orthopaedics and Traumatology
基金
广东省中药管局科研项目(编号:20203011)
广东省医学科学技术研究基金项目(编号:B2020030)。
