摘要
【目的】研究日粮添加甘露寡糖(Mannose oligosaccharide,MOS)对罗曼蛋鸡脂肪肝综合征及肠道菌群的影响。【方法】试验选取80只230日龄的罗曼蛋鸡,分为4组,每组20只。分别为普通日粮组(CK组),高脂日粮组(HFD组),高脂日粮+0.1%(w)甘露寡糖组(0.1%MOS组),高脂日粮+0.5%(w)甘露寡糖组(0.5%MOS组)。试验第9周采集蛋鸡血清、肝脏、腹脂及肠道内容物,通过血清指标、肝脏中相关基因表达分析及肠道微生物测序等,探讨甘露寡糖对蛋鸡脂肪肝发生的调控作用。【结果】高脂日粮显著提高了罗曼蛋鸡体质量、肝脏质量、肝脏指数、腹脂质量和腹脂指数,并诱发蛋鸡脂肪肝;甘露寡糖显著降低了高脂日粮引起的蛋鸡体质量、肝脏质量和腹脂质量增加;甘露寡糖显著抑制了高脂日粮引起的蛋鸡血清脂肪含量和肝脏中的脂肪沉积增加,抑制了肝脏中脂肪合成基因ACC、FAS和SCREBP-1c的mRNA表达水平,并缓解了蛋鸡脂肪肝;甘露寡糖显著提高了蛋鸡盲肠中拟杆菌门Bacteroidetes的相对丰度,显著降低了厚壁菌门Firmicutes的相对丰度。【结论】在高脂日粮中添加甘露寡糖能够改善蛋鸡肠道菌群,抑制肝脏中脂肪合成相关基因的mRNA表达水平,缓解高脂日粮引起的蛋鸡脂肪肝。饲粮中添加质量分数为0.5%的甘露寡糖对降低蛋鸡体质量、肝脏质量和腹脂质量的效果更好。
【Objective】The purpose of this study was to investigate the effect of dietary supplementation of mannose oligosaccharide(MOS)on fatty liver syndrome and intestinal flora of Roman laying hens.【Method】Eighty 230-day-old Roman laying hens were divided into four groups with 20 hens in each group,including the normal diet group(CK group),high-fat diet group(HFD group),HFD+0.1%MOS group(0.1%MOS group),and HFD+0.5%MOS group(0.5%MOS group).Samples of serum,liver,abdominal fat and intestinal contents were taken at the 9th week of the experiment.The regulatory effect of MOS on fatty liver in Roman hens was investigated by analyzing the serum indicators and gene expression in liver and gut microbiota sequencing. 【Result】High-fat diet significantly increased body weight, liver weight, liver index, abdominal fat weight and abdominal fat index of Roman laying hens, and induced fatty liver in laying hens. MOS significantly reduced the increase of body weight, liver weight and abdominal fat weight of laying hens caused by HFD. MOS significantly inhibited the increase of fat deposition in the serum and liver of laying hens caused by HFD. MOS inhibited mRNA expression levels of fat synthesis genes ACC, FAS and SCREBP-1c in liver, and alleviated fatty liver of laying hens. MOS significantly increased the relative abundance of Bacteroidetes and decreased the relative abundance of Firmicutes in cecum of laying hens.【Conclusion】Dietary supplementation of MOS in HFD can improve the gut microbiome of laying hens, and inhibit mRNA expression levels of genes related to fat synthesis in liver, thereby alleviating the fatty liver of laying hens caused by HFD. Addition of 0.5% MOS in diet has a better effect for reducing the body weight, liver weight and abdominal fat weight of laying hens.
作者
赵伟杰
冯晓华
梁競文
莫颖芬
朱灿俊
束刚
高萍
王松波
王丽娜
江青艳
ZHAO Weijie;FENG Xiaohua;LIANG Jingwen;MO Yingfen;ZHU Canjun;SHU Gang;GAO Ping;WANG Songbo;WANG Lina;JIANG Qingyan(College of Animal Science,South China Agricultural University/Guangdong Provincial Key Laboratory of Animal Nutrition Control,Guangzhou 510642,China)
出处
《华南农业大学学报》
CAS
CSCD
北大核心
2024年第1期15-22,共8页
Journal of South China Agricultural University
基金
国家自然科学基金(31790411)。
关键词
甘露寡糖
高脂日粮
罗曼蛋鸡
脂肪肝综合征
肠道菌群
Mannose oligosaccharide
High-fat diet
Roman laying hen
Fatty liver syndrome
Intestinal flora
