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Toll样受体和白细胞介素-17单核苷酸多态性与口腔扁平苔藓易感性的相关性研究

The correlation study between single nucleotide polymorphisms of Toll-like receptor and interleukin-17 and oral lichen planus
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摘要 目的探讨Toll样受体(TLR)和白细胞介素-17(IL-17)基因的遗传多态性及与口腔扁平苔藓(OLP)的相关性。方法选取病例组(OLP组)患者与对照组(未患OLP人群)外周血提取的DNA为研究对象,选取TLR与IL-17上的7个单核苷酸多态性(SNP)位点,分别是TLR3中的rs5743312、TLR2中的rs121917864和IL-17A中的5个SNP位点(rs4711998、rs3819024、rs8193036、rs3748067和rs17878530)。采用高分辨率熔解曲线分析法进行外周血细胞PCR产物基因多态性检测,并与DNA测序结果比对。统计分析SNP位点与OLP发生的相关性。结果rs5743312(TLR3)、rs3819024(IL-17A)与OLP的发生相关,并有统计学意义(P<0.05)。其余5个SNP位点未检测出与OLP相关。结论rs5743312(TLR3)和rs3819024(IL-17A)可能与OLP的易感性相关。 Objective This study aimed to explore the genetic polymorphisms of the Toll-like receptor(TLR)and interleukin(IL)-17 genes and their correlation with oral lichen planus(OLP).Methods DNA extracted from the peripheral blood of patients in the case(OLP group)and control(non-OLP population)groups were selected as the research subjects.Seven single nucleotide polymorphisms were attempted in our study,namely,rs5743312 in TLR3,rs121917864 in TLR2,and five others(rs4711998,rs3819024,rs8193036,rs3748067,and rs17878530)in IL-17A.High resolution melting curve analysis was used to detect the gene polymorphism of PCR products in peripheral blood cells and compared with DNA sequencing.The correlation between polymorphic sites and the occurrence of OLP was statistically analyzed.Results We found that rs5743312(TLR3)and rs3819024(IL-17A)had a statistically significant association with OLP(P<0.05).The remaining five SNPs cannot be tested for association with OLP.Conclusion rs5743312(TLR3)and rs-3819024(IL-17A)may contribute to the initiation of OLP.
作者 李然 胡月 张宁 高瑞芳 王翔宇 葛学军 Li Ran;Hu Yue;Zhang Ning;Gao Ruifang;Wang Xiangyu;Ge Xuejun(Dept.of Pediatric and Preventive Dentistry,Shanxi Medical University School&Hospital of Stomatology,Taiyuan 030001,China;Shanxi Medical University School&Hospital of Stomatology,Shanxi Province Key Laboratory of Oral Diseases Prevention and New Materials,Taiyuan 030001,China;Dept.of Pediatric Dentistry,Homony Long Stomatological Hospital,Taiyuan 030006,China;Dept.of Stomatology,Emergency General Hospital,Beijing 100028,China)
出处 《国际口腔医学杂志》 CAS CSCD 北大核心 2024年第1期45-51,共7页 International Journal of Stomatology
基金 山西省基础研究计划项目(202103021223235)。
关键词 单核苷酸多态性 高分辨率熔解曲线分析 口腔扁平苔藓 易感性 single nucleotide polymorphism high resolution curve melting analysis oral lichen planus susceptibility
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  • 1ZHANG Xiao-lin,PEI Fang,HAN Ya-Ling,YAN Cheng-Hui, HUANG Ming-Fang,WANG Tao (Department of Cardiology,Cardiovascular Institute of PLA, Shenyang Northern Hospital,Shenyang 110031,China).Interleukin-17A gene variants and risk of coronary artery disease:a large angiography-based study[J].岭南心血管病杂志,2011,17(S1):150-151. 被引量:8
  • 2Zhang SY, Herman M, Ciancanelli MJ, Perez de Diego R, Sancho-Shimizu V,Abel L, et al. TLR3 immunity to infection in mice and humans. Curr OpinImmunol. 2013;25:19–33.
  • 3Frazao JB, Errante PR, Condino-Neto A. Toll-like receptors’ pathwaydisturbances are associated with increased susceptibility to infections inhumans. Arch Immunol Ther Exp (Warsz). 2013;61:427–43.
  • 4Netea MG, Wijmenga C, O’Neill LA. Genetic variation in Toll-like receptorsand disease susceptibility. Nat Immunol. 2012;13:535–42.
  • 5He JF, Jia WH, Fan Q, Zhou XX, Qin HD, Shugart YY, et al. Geneticpolymorphisms of TLR3 are associated with nasopharyngeal carcinoma riskin Cantonese population. BMC Cancer. 2007;7:194.
  • 6Singh V, Srivastava N, Kapoor R, Mittal RD. Single-nucleotide polymorphismsin genes encoding Toll-like receptor-2, -3, -4, and -9 in a case-control studywith bladder cancer susceptibility in a North Indian population. Arch MedRes. 2013;44:54–61.
  • 7Mandal RK, George GP, Mittal RD. Association of Toll-like receptor (TLR) 2, 3and 9 genes polymorphism with prostate cancer risk in North Indianpopulation. Mol Biol Rep. 2012;39:7263–9.
  • 8Pandey S, Mittal B, Srivastava M, Singh S, Srivastava K, Lal P, et al. Evaluationof Toll-like receptors 3 (c.1377C/T) and 9 (G2848A) gene polymorphisms incervical cancer susceptibility. Mol Biol Rep. 2011;38:4715–21.
  • 9Etokebe GE, Knezevic J, Petricevic B, Pavelic J, Vrbanec D, Dembic Z.Single-nucleotide polymorphisms in genes encoding toll-like receptor-2,-3, -4, and -9 in case-control study with breast cancer. Genet Test MolBiomarkers. 2009;13:729–34.
  • 10Li G, Zheng Z. Toll-like receptor 3 genetic variants and susceptibility tohepatocellular carcinoma and HBV-related hepatocellular carcinoma.Tumour Biol. 2013;34:1589–94.

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