摘要
目的 研究葛根素对D-半乳糖诱导的心脏老化的保护作用。方法 24只6~8周龄的昆明小鼠随机分成对照组、D-半乳糖组、葛根素低剂量组和葛根素高剂量组4组,通过持续皮下注射D-半乳糖(150 mg/kg/d)建立衰老模型,造模成功后给予葛根素和等量0.9%氯化钠溶液灌胃,60 d后,应用HE染色检测心肌组织形态学改变;采用自动生化分析仪检测血清心肌肌钙蛋白T(cardiac troponin T,c Tn T)和肌酸激酶(creatine kinase,CK);应用ELISA法检测丙二醛(malondialdehyde,MDA)含量和超氧化物歧化酶(superoxide dismutase,SOD)活性;使用二氢乙锭(Dihydroethidium,DHE)荧光探针测量心肌组织中的组织活性氧(reactive oxygen species,ROS)水平;免疫组织化学法检测组织8-羟基脱氧鸟苷(8-hydroxydeoxyguanosine,8-OHdG)表达,蛋白印迹法检测4-羟基-2-壬烯醛(4-hydroxy-2-nonenal,4-HNE)、p21和p53蛋白表达。结果 与对照组比较,D-半乳糖组小鼠心肌纤维排列不规则,c Tn T、CK、ROS、MDA的水平升高、SOD的水平降低,8-OHdG、4-HNE、p21和p53表达水平明显升高(P<0.01)。与D-半乳糖组比较,葛根素低剂量和葛根素高剂量给药组小鼠血清中c Tn T、CK水平明显降低(P<0.05)。此外,心肌组织ROS、丙二醛(malondialdehyde,MDA)、8-OHdG、4-HNE、p21和p53表达水平也明显降低,超氧化物歧化酶(superoxide dismutase,SOD)的水平显著增加(P<0.05)。结论 葛根素通过抑制氧化应激减弱D-半乳糖诱导的心脏衰老,表明葛根素可作为抗衰老治疗的潜在有效中草药活性成分。
Objective To investigate the protective effects of puerarin on D-galactose(D-gal)induced heart aging.Methods 24 Kunming mice aged 6-8 week old were randomly divided into normal control group,D-gal treated group,low-dose puerarin-treated group and high-dose puerarin-treated group.The aging model was established by continuous subcutaneous injection of D-gal(150 mg/kg/d),puerarin and the same amount of 0.9%sodium chloride solution were given intragastric administration after successful modeling.After 60 days,HE staining was performed to observe morphological changes of myocardial tissues,automatic biochemical analyzer was used to detect serum biochemical parameters including the cardiac troponin T(cTnT)and creatine kinase(CK),malondialdehyde(MDA)content and superoxide dismutase(SOD)activity were measured by ELISA,fluorescence probe dihydroethidium(DHE)were used to detect the levels of reactive oxygen species(ROS),immunohistochemical method was used to detect the expression of 8-hydroxydeoxyguanosine(8-OHdG),Western blot was used to detect the expressions of 4-hydroxy-2-nonenal(HNE),p21 and p53 in myocardial tissues.Results Compared with control group,irregularly arranged cardiac muscle fibers were observed in myocardial tissues of D-gal-treated mice,the levels of cTnT,CK,ROS and MDA were upregulated,and the SOD activity was significantly decreased,the expressions of 8-OHdG,4HNE,p21,and p53 was upregulated after D-gal treatment(P<0.01).Compared with D-gal treated group,serum cTnT and CK levels in low-dose puerarin-treated group and high-dose puerarin-treated group were significantly decreased(P<0.05).In addition,the expression levels of ROS,MDA,8-OHdG,4-HNE,p21 and p53 in myocardial tissues were significantly decreased,while the level of SOD was significantly increased(P<0.05).Conclusion Puerarin might serve as a potential effective Chinese herbal active constituent for anti-aging therapy.
作者
宋冲
杜青
李心亮
SONG Chong;DU Qing;LI Xinliang(Medical School,Changsha Social Work College,Changsha 410004,China;Institute of Chinese Medicine,Hunan Academy of Chinese Medicine,Changsha 410006,China)
出处
《中国中医基础医学杂志》
CAS
CSCD
2024年第1期35-40,共6页
JOURNAL OF BASIC CHINESE MEDICINE
基金
湖南省自然科学基金项目(2020JJ7090)
湖南省教育厅科学研究项目优秀青年项目(21B0931)。
