摘要
K^(+)/H^(+)逆向转运蛋白通过调节细胞内K^(+)和pH稳态,在植物的营养、生长、发育和信号转导中发挥重要作用。在本研究中,利用全基因组分析5种十字花科植物拟南芥(Arabidopsis thaliana)、琴叶拟南芥(Arabidopsis lyrata)、小鼠耳芥(Arabidopsis pumila)、亚麻荠(Camelina sativa)和盐芥(Eutrema salsugineum)分别鉴定出6、6、12、18和6个KEA基因。系统进化、基因结构和motif分析表明以上48个KEA基因隶属于CladeⅠ和CladeⅡ两个亚家族。共线性分析发现,在小鼠耳芥和亚麻荠进化中,KEA家族成员既经历了全基因组复制同时又经历了片段重复事件,而拟南芥、琴叶拟南芥和盐芥中大部分KEA成员以经历分散型复制事件为主。比较进化分析表明5种植物KEA家族的旁系同源基因在进化中的主要动力为纯化选择。物种间KEA基因的共线性分析表明十字花科物种之间存在复杂的进化关系。小鼠耳芥KEA基因的表达明显响应高盐胁迫,其中ApKEA2.1/2.2、ApKEA6.1/6.2的表达模式存在明显差异,暗示复制基因对功能发生分歧。本研究是对植物中K^(+)/H^(+)逆向转运蛋白的新认识,研究结果为进一步研究K^(+)/H^(+)平衡与钾素动态营养机制提供了基因资源和理论基础。
K^(+) efflux antiporter plays an important role in intracellular K^(+) and pH homeostasis,and function in plant nutrition,growth,development and signal transduction.In this study,genome-wide analysis identified 6,6,12,18 and 6 KEA genes in five Brassicaceae plants,Arabidopsis thaliana,Arabidopsis lyrate,Arabidopsis pumila,Camelina sativa and Eutrema salsugineum,respectively.Phylogenetic evolution,gene structure,and motif analyses showed that 48 KEA genes were classified into Clade Ⅰ and Clade Ⅱ subfamilies.Collinearity analysis revealed that during the evolution of A.pumila and C.sativa,the KEA family members experienced both whole-genome duplication and segmental duplication events,while most of the KEA members in A.thaliana,A.lyrata and E.salsugineum were mainly dispersed duplication events.Comparative evolutionary analysis demonstrated that purification selection was the main driving force for the evolution of the KEA paralogous genes in five plants.Collinearity analysis of KEA genes among species indicated a complex evolutionary relationship between Brassicaceae species.The expression of ApKEA gene s significantly respond to high salt stress.In addition,the expression patterns of ApKEA2.1/2.2 and ApKEA6.1/6.2 were significantly different,indicating that they functionally diverged.The results of this study expanded our understanding of the K^(+)/H^(+) antiporters in plants,and provided genetic resources and theoretical bases for further analysis of K^(+)/H^(+) balance and potassium dynamic nutrition mechanism.
作者
郭力
金玉环
刘丹青
李永光
黄先忠
Guo Li;Jin Yuhuan;Liu Danqing;Li Yongguang;Huang Xianzhong(Special Plant Genomics Laboratory,College of Life Sciences,Shihezi University,Shihezi,832003;Center for Crop Biotechnology,College of Agriculture,Anhui Science and Technology University,Fengyang,233100)
出处
《分子植物育种》
CAS
北大核心
2024年第1期48-60,共13页
Molecular Plant Breeding
基金
国家自然科学基金项目(U1303302)
石河子大学国际合作项目(GJHZ201806)
安徽科技学院学科带头人引进人才启动经费项目(NXYJ202001)共同资助。
